| 棉铃虫Gq蛋白α亚基基因的克隆及组织特异性表达 |
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| 引用本文： | 苏宏华,王桂荣,吴孔明,郭予元.棉铃虫Gq蛋白α亚基基因的克隆及组织特异性表达[J].中国农业科学,2006,39(4):734-740. |
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| 作者姓名： | 苏宏华 王桂荣 吴孔明 郭予元 |
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| 作者单位： | 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京,100094 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 【目的】了解棉铃虫体内Gqα的组织特异性表达情况。【方法】利用RACE技术获得了Gqα全长基因，利用半定量RT-PCR研究了该基因的时空表达情况。【结果】从棉铃虫Helicoverpa armigera触角中克隆了一条全长1 101bp的Gqα cDNA序列，命名为GqαHarm；阅读框全长1 062 bp，编码353个氨基酸残基。GqαHarm与已报道的昆虫Gq蛋白α亚基同源性在90％以上，与近缘种甘蓝夜蛾Mamestra brassicae Gqα的同源性高达96.88％，与家蚕Bombyx mori Gqα的同源性高达97.73％。半定量RT-PCR研究表明，GqαHarm在棉铃虫雌雄成虫触角中表达，在头、胸、腹、足和翅中也有表达，并且表达量差异不显著；此外，在喙、下颚须和下唇须中也有表达；在卵、幼虫、蛹和成虫体内也都有表达；在卵中的表达量最高，而在成虫中的表达量最低，在幼虫和蛹的前、中、后期的表达量差异不大。【结论】研究表明GqαHarm是一种在棉铃虫体内普遍存在的蛋白。
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| 关 键 词： | 棉铃虫 Gq蛋白α亚基 基因克隆 表达谱 |
| 收稿时间： | 2005-10-26 |
| 修稿时间： | 2005-10-262006-02-05 |
Gene Cloning and Tissue-Specific Expression of Gqα in Helicoverpa armigera |
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| SU Hong-hua,WANG Gui-rong,WU Kong-ming,GUO Yu-yuan.Gene Cloning and Tissue-Specific Expression of Gqα in Helicoverpa armigera[J].Scientia Agricultura Sinica,2006,39(4):734-740. |
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| Authors: | SU Hong-hua WANG Gui-rong WU Kong-ming GUO Yu-yuan |
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| Institution: | State Key Laboratory for Biology of Plant Disease and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094 |
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| Abstract: | 【Objective】In order to find out the tissue-specific expression of Gqα in Helicoverpa armigera, 【Method】a full-length cDNA, Gqα, was cloned by RACE and named as GqαHarm. And its spatio-temporal expression pattern was studied by Semi-quantitative RT-PCR analysis. 【Result】 GqαHarm was obtained from the antennae. And its open reading-frame was 1062bp, encoding 353 amino acid residues. GqαHarm shares high identity (>90%) with reported Gqα from other insects and the homology with Bombyx mori and its close sib population Mamestra brassicae reached 97.73% and 96.88%, respectively. Semi-quantitative RT-PCR analysis showed that Gqα was not only expressed in antenna, but also in the head without antenna, thorax, leg and wing of H. armigera. There was no obvious difference in the expression in tissues. Moreover, it was also expressed in labial palpus, maxillary palpus and proboscis. Gqα was expressed in egg with a highest level, and in imago with a lowest level; and there was no significant diffenrence in the expression at different stages for the larva and the pupae. 【Conclusion】The spacial and temporal expression pattern indicated that GqαHarm was a ubiquitous protein in Helicoverpa armigera . |
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| Keywords: | Helicoverpa armigera Gq protein alpha subunit Gene cloning Expression pattern |
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