| 草莓内生细菌的分离及草莓灰霉病菌拮抗菌的筛选鉴定 |
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| 引用本文： | 李娜,戴美学.草莓内生细菌的分离及草莓灰霉病菌拮抗菌的筛选鉴定[J].植物保护,2010,36(4):70-74. |
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| 作者姓名： | 李娜 戴美学 |
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| 作者单位： | 山东师范大学生命科学学院,济南,250014 |
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| 摘 要： | 本研究从健康的草莓植株内分离到54株内生细菌,其中根、茎、叶组织中分离内生细菌种群密度为2.8×101～3.95×104cfu/g鲜重不等。通过对峙试验,得到5株对草莓灰霉病菌有强拮抗作用的菌株,占所分离内生细菌总数的9.26%。来自草莓叶的SL6菌株抑菌效果最佳,抑菌半径达15mm,无菌发酵液对草莓灰霉病菌菌丝的抑制率为97.6%,具有作为生防菌的应用潜能。经形态、生理生化和16SrRNA等分析测定,并通过MEGA4方法构建其16SrDNA系统发育树,将SL6菌株鉴定为枯草芽胞杆菌(Bacillus subtilis)。
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| 关 键 词： | 草莓 内生细菌 灰霉病菌 拮抗菌 |
Isolation of endophytic bacteria from strawberry and identification of antagonistic bacteria to Botrytis cinerea in strawberry |
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| Li N,Dai Meixue.Isolation of endophytic bacteria from strawberry and identification of antagonistic bacteria to Botrytis cinerea in strawberry[J].Plant Protection,2010,36(4):70-74. |
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| Authors: | Li N Dai Meixue |
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| Institution: | College of Life Sciences, Shandong Normal University, Ji’nan 250014, China |
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| Abstract: | Fifty four endophytic strains were isolated from different internal tissues of strawberry plants. The results indicated that the population densities of endophytic bacteria ranged from 2.8×101-3.95×104 cfu/g fresh weight, although it varied depending on tissues of the plant. Five strains, accounting for about 9.26% of the endophytic bacteria, exhibited antagonistic activities against pathogenic fungi, among which the strain SL6 isolated from the leaves of strawberry was found to have the best control efficacy, with an inhibition zone diameter of 15 mm and 97.6% inhibitory rate with the fermentation filtrate, revealing potential in antagonistic action. Based on the morphological, physiological and biochemical characteristics and 16S rRNA sequence analysis, SL6 was identified as Bacillus subtilis. |
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| Keywords: | strawberry endophytic bacteria Botrytis cinerea antagonistic bacteria |
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