Detection of a mammalian-like group A rotavirus in diarrhoeic chicken |
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| Authors: | Wani S A Bhat M A Ishaq S M Ashrafi M A Buchh A S Haq M |
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| Institution: | Division of Veterinary Microbiology and Immunology, SK University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shuhama, (Alusteng) Srinagar 190006, India. microlab@sancharnet.in |
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| Abstract: | The present investigation describes detection of a mammalian-like electropherogroup A rotavirus in chicken with diarrhoea. This also records the first detection of a rotavirus in an avian species from India. During the investigation 75 diarrhoeic faecal samples collected from adult chicken were screened for the presence of group A rotavirus antigen by sandwich ELISA. All three samples positive for rotavirus antigen revealed 11 bands of RNA in polyacrylamide gel electrophoresis (PAGE). In contrast to avian group A rotavirus, segment 5 was found to migrate closer to 6 as is the case with mammalian group A rotaviruses. Segments 7, 8 and 9 were found to migrate as a tight triplet, which is characteristic of group A rotavirus. |
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