| 外源过氧化氢对干旱胁迫下温室黄瓜叶绿体超微结构和抗氧化酶的影响 |
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| 引用本文： | 刘忠静,郭延奎,林少航,白吉刚.外源过氧化氢对干旱胁迫下温室黄瓜叶绿体超微结构和抗氧化酶的影响[J].园艺学报,2009,36(8):1140-1146. |
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| 作者姓名： | 刘忠静 郭延奎 林少航 白吉刚 |
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| 作者单位： | (山东农业大学生命科学学院, 作物生物学国家重点实验室, 山东泰安271018) |
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| 基金项目： | 作物生物学国家重点实验室开放课题 |
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| 摘 要： | 以温室黄瓜品种‘春光2号’为试材, 用100 g·L - 1聚乙二醇处理模拟干旱胁迫, 研究外源过氧化氢是否通过提高叶片抗氧化酶的活性来减轻干旱胁迫对叶绿体膜的伤害。结果表明: 干旱胁迫处理48 h后, 叶绿体肿胀变圆, 叶绿体膜和类囊体模糊不清。与干旱胁迫前没有经过预处理的幼苗相比, 经外源过氧化氢预处理后再干旱胁迫48 h的幼苗中大部分的叶绿体结构正常, 叶片的超氧化物歧化酶、过氧化氢酶、愈创木酚过氧化物酶、抗坏血酸过氧化物酶、单脱氢抗坏血酸还原酶、脱氢抗坏血酸还原酶和谷胱苷肽还原酶等抗氧化物酶的活性以及抗坏血酸和还原型谷胱苷肽的含量明显增高, 内源过氧化氢含量、超氧阴离子的产生速率和丙二醛的含量则显著降低。所以, 外源过氧化氢预处理能够提高抗氧化物的活性,降低活性氧的积累, 从而缓解干旱对黄瓜叶绿体膜的伤害。
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| 关 键 词： | 黄瓜 过氧化氢 干旱 超微结构 抗氧化物 |
| 收稿时间： | 2009-2-4 |
| 修稿时间： | 2009-6-19 |
Effects of Exogenous Hydrogen Peroxide on Ultrastructure of Chloroplasts and Activities of Antioxidant Enzymes in Greenhouse-ecotype Cucumber Under Drought Stress |
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| LIU Zhong-jing,GUO Yan-kui,LIN Shao-hang,BAI Ji-gang.Effects of Exogenous Hydrogen Peroxide on Ultrastructure of Chloroplasts and Activities of Antioxidant Enzymes in Greenhouse-ecotype Cucumber Under Drought Stress[J].Acta Horticulturae Sinica,2009,36(8):1140-1146. |
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| Authors: | LIU Zhong-jing GUO Yan-kui LIN Shao-hang BAI Ji-gang |
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| Institution: | (State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, Shandong 271018,China) |
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| Abstract: | To investigate whether exogenous-H2O2-induced drought tolerance is mediated by the increasing of antioxidant activities, agreenhouse-ecotype cucumber ‘Chunguang 2 ’was pretreated with exogenous hydrogen peroxide (H2O2 ) and was subjected to drought stress, which was simulated by 100 g·L - 1 polyethylene glycol 6000. Without pretreatment of exogenous H2O2 , drought stress resulted in plants with round chlorop lasts, the indistinct chlorop lastmembranes and thylakoids. Compared to drought treatment itself, the combination of H2O2 pretreatment and drought stress did not change the chlorop last ultrastructure obviously, and it not only increased the activities of antioxidants such as superoxidedismutase, catalase, guaiacol peroxidase, ascorbate peroxidase, monodehydroascorbate reductase, dehydrateascorbate reducatase, glutathione reductase,ascorbic acid and the reduced glutathione, but also decreased the contents of malonaldehyde, endogenous H2O2 and superoxide radical. We conclude that the app lication of exogenous H2O2 increases the antioxidant activities, and therefore decreases the accumulation of reactive oxygen species and alleviates the damage to chlorop lastmembranes under drought stress. |
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| Keywords: | cucumber hydrogen peroxide drought ultrastructure antioxidant |
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