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Identification of endophytic <Emphasis Type="Italic">Streptomyces</Emphasis> sp. R-5 and analysis of its antimicrobial metabolites
Authors:Masafumi?Shimizu  Yasuhiro?Igarashi  Tamotsu?Furumai  Hiroyasu?Onaka  Email author" target="_blank">Hitoshi?KunohEmail author
Institution:(1) Laboratory of Ecological Circulation, Faculty of Bioresources, Mie University, Tsu, 514-8507, Japan;(2) Biotechnology Research Center, Toyama Prefectural University, Toyama, Japan
Abstract:In a previous study, endophytic Streptomyces sp. R-5 isolated from a field-grown rhododendron was able to confer disease resistance on tissue-cultured seedlings of rhododendron when applied to medium surfaces in flasks. Here, the isolate was identified as Streptomyces galbus Frommer based on various physiological characteristics and analysis of the 16S rDNA sequence. Its major antimicro-bial metabolites were identified as actinomycin X2 and fungichromin by analyses using liquid chromatography/mass spectometry and nuclear magnetic resonance.
Keywords:Endophytic  Streptomyces galbus  Biocon-trol  Actinomycin X2  Fungichromin  Rhododendron
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