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菊花营养生长期观赏性状的RAPD和ISSR标记
引用本文:张飞,陈发棣,房伟民,陈素梅.菊花营养生长期观赏性状的RAPD和ISSR标记[J].园艺学报,2010,37(8):1345-1350.
作者姓名:张飞  陈发棣  房伟民  陈素梅
作者单位:(南京农业大学园艺学院,南京 210095)
基金项目:国家自然科学基金项目 
摘    要:(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)

关 键 词:菊花  营养生长期  数量性状  RAPD  ISSR  方差分析  
收稿时间:2009-12-23

Detection of RAPD and ISSR Markers Associated with Ornamental Traits of Chrysanthemum in Vegetative Stage
ZHANG Fei,CHEN Fa-di,FANG Wei-min,CHEN Su-mei.Detection of RAPD and ISSR Markers Associated with Ornamental Traits of Chrysanthemum in Vegetative Stage[J].Acta Horticulturae Sinica,2010,37(8):1345-1350.
Authors:ZHANG Fei  CHEN Fa-di  FANG Wei-min  CHEN Su-mei
Abstract:Association analysis were undertaken to detect the genetic markers significantly related to the 6 ornamental traits(i.e. plant height,plant width,the ratio of plant height to plant width,internode length,leaf length and leaf width)in vegetative stage by the method of One-Way ANOVA based on a F1 population derived from chrysanthemum cultivars,‘Yuhualuoying’and ‘Aoyunhanxiao’. The result showed that the 6 traits segregated significantly in the F1 population(coefficient of variation > 20%),basically fitting in a normal distribution. The One-Way ANOVA analysis revealed that the number of genetic markers significantly associated with plant height,plant width,the ratio of plant height to plant width,internode length,leaf length and leaf width was 15,9,9,11,7 and 8,respectively. Of these genetic markers,the cumulative contribution ratio for each trait was above 30%,whereas the contribution ratio of each single genetic marker ranged from 2.77% to 8.50%,revealing that these markers were polygene with little effect. We anticipated that the proper utilization of these genetic markers significantly associated with these target traits would effectively improve selection breeding in chrysanthemum.
Keywords:RAPD  ISSR
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