| ABC─Dot─ELISA快速检测食品中沙门氏菌 |
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| 引用本文： | 任家琰,魏荣,余晓琼,耿丹.ABC─Dot─ELISA快速检测食品中沙门氏菌[J].山西农业大学学报(自然科学版),1995(1). |
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| 作者姓名： | 任家琰 魏荣 余晓琼 耿丹 |
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| 作者单位： | 山西农业大学动医系，山西晋中农业学校 |
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| 摘 要： | 本试验采用生物素—亲和素酶复合物—斑点酶联免疫吸附试验（ＡＢＣ—Ｄｏｔ—ＥＬＩＳＡ）检测食品中沙门氏菌。该法可检出ＩＳＣ液中接种的沙门氏菌。其敏感性可达１０４—１０５千个／Ｌ，比常规免疫酶法和免疫荧光法提高１０—１００倍。特异性检测表明，其与大肠杆菌、变形杆菌、产气杆菌未发生交叉反应。该法在２４ｈ内可报告结果，比常规法至少快３—４ｄ。对２００份食品检样进行检测，检出１９份，检出率９．５％，与常规法检测结果平行比较，漏检率仅为０．５％，结果相差不显著（Ｐ＞０．０５）。进行３次重复性试验，结果完全相同。试验结果表明，该法操作简便、敏感、快速，具有较高的特异性，适于对大批样品进行快速检测。
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| 关 键 词： | ABC—Dot—ELISA快速检测，沙门氏菌，食品 |
DETECTION OF SALMONELLA IN FOOD BY ABC-Dot-ELISA |
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| Ren Jiayan et al..DETECTION OF SALMONELLA IN FOOD BY ABC-Dot-ELISA[J].Journal of Shanxi Agricultural University,1995(1). |
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| Authors: | Ren Jiayan |
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| Abstract: | Salmonella in food were detected by using of ABC-Dot-ELISA. The bacteria cultured in ISC solution could be detected with the method. The sensitivity of the method reached 104 105 bacteria/1ml. which is 10-100 times and 10 times higher thanthose of Dot-ELISA and Fluorescent Antibody technique respectively. Speciality testing revealed no cross-reactions between the bacteria and E. colt, B. gasoformans. The method giving results within 24 hours was 3-4 days faster than common method. Of 200 food samples, 19 were positive. The positive rate 9. 5 percent. Compared with the common method, the difference of the results was not significant (P>0.05). The repeatability of the results was good. Therefore, the method is simple, sensitive, rapid and specific and can be used f6r rapid detection of a great quantity of samples. |
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| Keywords: | ABC-Dot-ELISA Salmonella Food |
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