| 抗生素降低彩色马蹄莲组织培养污染的研究 |
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| 引用本文： | 周凤娇,巫华美,陈训.抗生素降低彩色马蹄莲组织培养污染的研究[J].种子,2010,29(12). |
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| 作者姓名： | 周凤娇 巫华美 陈训 |
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| 作者单位： | 贵州省林业科学研究院;贵州省生物研究所;贵州科学院; |
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| 基金项目： | 贵州省农业科技攻关项目 |
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| 摘 要： | 以生长于土壤中易污染的马蹄莲一年生小块茎为试材,在培养基中添加硫酸庆大霉素、青霉素、硫酸链霉素3种抗生素,浓度梯度为25、50 mg/L和75 mg/L进行初代培养进行组织培养,未添加抗生素作为对照。结果表明:3种抗生素都可以明显降低污染率,而以硫酸链霉素抑菌效果最好。特别是浓度为50 mg/L的链霉素抑菌效果最明显。
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| 关 键 词： | 抗生素 彩色马蹄莲 初代培养 污染 |
Study on the Effect of Several Antibiotics in Reducing Pollution of Zantedeschia Hvbrida Tissue Culture |
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| ZHOU Feng-Jiao,WU Hua-mei,CHEN Xun.Study on the Effect of Several Antibiotics in Reducing Pollution of Zantedeschia Hvbrida Tissue Culture[J].Seed,2010,29(12). |
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| Authors: | ZHOU Feng-Jiao WU Hua-mei CHEN Xun |
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| Institution: | ZHOU Feng-Jiao1,WU Hua-mei2,CHEN Xun3(1.Forestry Research Institute of Guizhou Province,Guiyang 550005,China,2.Institute of Biology,Guizhou Province,Guiyang 550009,3.Guizhou Academy of Sciences,Guiyang 550001,China) |
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| Abstract: | Some small tubers of annual of Zantedeschia,growing in the soil pollution were cultured in the medium of added Gentamicin,penicillin,streptomycin,which concentration gradient were 25mg/L,50mg/L and 75mg/L,without addition of antibiotics as control.The results showed that Three kinds of antibiotics could significantly reduce the contamination rate,while the inhibitory effect of streptomycin sulfate was the best,especially in 50mg/L treatment. |
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| Keywords: | antibiotics Zantedeschia hvbrida primary culture pollution |
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