| 哺鸡竹亲缘关系的AFLP和SRAP分析 |
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| 引用本文： | 娄永峰,林新春,何奇江,郭小勤,黄丽春,方伟.哺鸡竹亲缘关系的AFLP和SRAP分析[J].分子植物育种,2010,8(1):83-88. |
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| 作者姓名： | 娄永峰 林新春 何奇江 郭小勤 黄丽春 方伟 |
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| 作者单位： | 1. 浙江林学院,森林培育技术重点实验室,临安,311300
2. 浙江省林业科学研究院,杭州,310023 |
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| 基金项目： | 浙江省重大科技专项(2006C12008)资助 |
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| 摘 要： | 本研究采用AFLP和SRAP分子标记技术对9个哺鸡竹类竹种(含2个变型)的亲缘关系进行分析。研究结果表明:12对AFLP引物共扩增出359条带,多态性条带比率为49.7%,相似系数变化范围在0.779～0.978之间;24对SRAP引物共扩增出258条带,多态性条带比率为64.0%,相似系数变化范围在0.721～0.958之间。UPGMA聚类分析表明,AFLP和SRAP标记均将哺鸡竹分成2大类:第Ⅰ类为富阳乌哺鸡竹、花哺鸡竹、黄纹竹、黄秆乌哺鸡竹、毛壳花哺鸡竹、乌哺鸡竹、白哺鸡竹;第Ⅱ类为云和乌哺鸡竹、红哺鸡竹。对这两种标记相似性系数进行相关性分析,AFLP与SRAP的结果呈极显著相关(r=0.934),但SRAP标记比AFLP标记可在竹类及其种下等级检测到更大的遗传变异。
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| 关 键 词： | 哺鸡竹 AFLP SRAP 亲缘关系 |
Analysis on Genetic Relationship of Puji-Bamboo Species by Using AFLP and SRAP |
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| Lou Yongfeng,Lin Xinchun,He Qijiang,Guo Xiaoqin,Huang Lichun,Fang Wei.Analysis on Genetic Relationship of Puji-Bamboo Species by Using AFLP and SRAP[J].Molecular Plant Breeding,2010,8(1):83-88. |
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| Authors: | Lou Yongfeng Lin Xinchun He Qijiang Guo Xiaoqin Huang Lichun Fang Wei |
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| Institution: | Lou Yongfeng Lin Xinchun He Qijiang Guo Xiaoqin Huang Lichun Fang Wei Key Laboratory for Modern Silvicultural Technology,Zhejiang Forestry University,Lin'an,311300,Zhejiang Forestry Academy,Hangzhou,310023 |
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| Abstract: | In this research,amplified fragment length polymorphisms(AFLP) and sequence-related amplified polymorphisms(SRAP) molecular markers were used to detect the genetic relationship among nine Puji-bamboo species,in which there were 2 cultivars,Ph.vivax f.huangwenzhu and Ph.vivax f.aureocaulis.359 bands were generated by 12 AFLP primer combinations with polymorphic bands accounting for 49.7% and similarity coefficient ranging from 0.779 to 0.978,and 258 bands were produced by 24 SRAP primer combinations with pol... |
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| Keywords: | Puji-bamboo AFLP SRAP Genetic relationship |
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