| 金冠×四倍体嘎拉杂种F_1代的倍性分析 |
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| 引用本文： | 田义,康国栋,杨振英,张彩霞,张利益,丛佩华.金冠×四倍体嘎拉杂种F_1代的倍性分析[J].新疆农业科学,2010,47(4):719-721. |
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| 作者姓名： | 田义 康国栋 杨振英 张彩霞 张利益 丛佩华 |
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| 作者单位： | 1. 中国农业科学院果树研究所,辽宁兴城,125100;国家苹果育种中心,辽宁兴城,125100
2. 中国农业科学院果树研究所,辽宁兴城,125100 |
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| 基金项目： | 国家 "863"项目，国家苹果产业技术体系，国家科技支撑项目 |
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| 摘 要： | 【目的】对金冠×四倍体嘎拉杂种F1代的倍性组成进行分析,以便鉴定出三倍体新种质;同时,为今后多倍体育种的亲本选择提供借鉴。【方法】以田间F1代植株茎尖幼嫩叶片为试材,利用流式细胞仪对杂种F1代个体进行了分析。【结果】32株的倍性得到了确认:包括三倍体27株,二倍体4株,单倍体1株,未发现有四倍体植株。三倍体比率高达84.4%,二倍体和单倍体的比率分别为12.5%和3.1%。【结论】金冠×四倍体嘎拉杂交组合是一个优良的创制三倍体新品种/种质的组合,今后可以利用其进行更大规模的杂交育种。
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| 关 键 词： | 苹果 杂交 三倍体 单倍体 |
Ploidy Analysis of Hybrid F1 Individuals through Golden Delicious×Tetraploid Gala |
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| TIAN Yi,KANG Guo-dong,YANG Zhen-ying,ZHANG Cai-xia,ZHANG Li-yi,CONG Pei-hua.Ploidy Analysis of Hybrid F1 Individuals through Golden Delicious×Tetraploid Gala[J].Xinjiang Agricultural Sciences,2010,47(4):719-721. |
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| Authors: | TIAN Yi KANG Guo-dong YANG Zhen-ying ZHANG Cai-xia ZHANG Li-yi CONG Pei-hua |
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| Institution: | TIAN Yi1,2,KANG Guo-dong1,YANG Zhen-ying1,ZHANG Cai-xia1,ZHANG Li-yi1,CONG Pei-hua1,2(1.Institute of Pomology,CAAS,Xingcheng Liaoning 125100,China,2.National Center for Apple Breeding,China) |
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| Abstract: | 【Objective】In this study,the objective is to obtain triploid apple germplasm by analyzing the ploidy of hybrid F1 individuals through Golden Delicious × Tetroploid Gala,and provide reference of parents selection.【Method】Flow Cytometry was used to determine the ploidy of young leaves of F1 individuals in field.【Result】Thirty two individuals were identified,including 4 diploid,27 triploid and 1 haploid individuals,but there were not tetroploid individuals appearing among F1 individuals.The rate of triploid individuals was up to 84.4%,the rate of diploid and haploid was 12.5% and 3.1% respectively.【Conclusion】The combination of'Golden Delicious'×'tetraploid Gala' was a good cross combination for creating triploid cultivars/germplasm,which could be used to breed on a large scale. |
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| Keywords: | apple crossing triploid haploid |
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