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| 东北地区玉米弯孢菌的致病性分化与RAPD分析 | |
| 引用本文： | 张欣芳,苏前富,宋淑云,杜俊岐,刘影,张伟,李红,晋齐鸣.东北地区玉米弯孢菌的致病性分化与RAPD分析[J].玉米科学,2010,18(6):121-125. |
| 作者姓名： | 张欣芳 苏前富 宋淑云 杜俊岐 刘影 张伟 李红 晋齐鸣 |
| 作者单位： | 1. 吉林农业大学,长春,130118;吉林省农业科学院植物保护研究所,吉林公主岭,136100 2. 吉林省农业科学院植物保护研究所,吉林公主岭,136100 3. 吉林农业大学,长春,130118 |
| 基金项目： | 国家粮食丰产工程项目(2006BAD02A16-3)、国家科技支撑计划项目(2006BAD08A06)、吉林省科技发展计划项目(2006BAD02A10-5-3)、国家玉米产业体系技术项目 |
| 摘 要： | 在东北三省15个地区采集分离26个玉米弯孢菌菌株,通过鉴别寄主技术与RAPD分子标记技术进行致病性与遗传分化的研究。结果表明,东北地区玉米弯孢菌在致病性与遗传上都存在明显的变异,二者有一定的相关性,未发现变异程度与菌株地理来源有明显的直接关系。26个菌株的致病性分为5种类型,其中中等致病类型分布遍及东北三省,为优势致病类群;强致病类型分布在吉林省的白城市;弱致病类型主要分布在吉林省的白山和梨树。用8个引物对供试菌株进行RAPD扩增,共获得77个RAPD标记,其中多态性标记71个,多态性比例92.2%。供试菌株在相似系数约0.71处共聚为4组,大多数致病性较强的菌株聚在一起,而弱致病株在相似系数相对较低时和其他菌株聚在一起。 |
| 关 键 词： | 玉米弯孢菌 致病性分化 RAPD |
| 收稿时间： | 3/5/2010 12:00:00 AM |
Pathogenicity Differentiation and RAPD Analysis of Curvularia Lunata in Northeast China |
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| ZHANG Xin-fang,SU Qian-fu,LI Hong,JIN Qi-ming,et al..Pathogenicity Differentiation and RAPD Analysis of Curvularia Lunata in Northeast China[J].Journal of Maize Sciences,2010,18(6):121-125. | |
| Authors: | ZHANG Xin-fang SU Qian-fu LI Hong JIN Qi-ming |
| Abstract: | 26 isolates of curvularia lunata, mainly isolated from corn curvularia leaf spot in 15 different geographic regions in Jilin, Liaoning and Heilongjiang province, which were characterized by differential host and random amplified polymorphic DNA(RAPD). The results indicated that there was abundant genetic diversity and pathogenicity differentiation in these isolates. Two kinds of variation may have a certain correlation. No close correlation was found between genetic diversity and geographic orgins of isolates. The strains for test were identified as five pathogenic types by differential host which higher pathogenic types was distributed widely in Northeast, therefore that could be considered as the dominant pathogenic group. However highest pathogenic types was distributed in Baicheng in Jilin Province, weakly pathogenic types was mainly distributed in Baishan and Lishu in Jilin Province. Eight primers were employed for RAPD analyses of 26 isolates. Out of the 77 RAPD markers, 71 polymorphic markers(P=92.2%) were obtain. All isolates were clustered into four distinct groups at similar level of 0.71. Most strains with highest and higher pathogenicity was clustered together, however strain with weak pathogenicity was clustered at lower similar level. |
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