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| 茶树菇超高压保鲜过程中一株类芽孢杆菌的鉴定及系统发育分析 | |
| 引用本文： | 许峰,刘宇,赵爽,王贺祥,张国庆,王守现,闫跃龙,侯宇,冯杉杉.茶树菇超高压保鲜过程中一株类芽孢杆菌的鉴定及系统发育分析[J].中国农学通报,2011,27(19):186-192. |
| 作者姓名： | 许峰 刘宇 赵爽 王贺祥 张国庆 王守现 闫跃龙 侯宇 冯杉杉 |
| 作者单位： | 1. 北京市农林科学院植物保护环境保护研究所,北京,100097 2. 中国农业大学农业生物技术国家重点实验室,北京,100193 3. 北京农学院生物技术学院/农业部都市农业(北方)重点实验室,北京,102206 |
| 基金项目： | 北京农学院基础基金“抗草莓灰霉芽孢杆菌筛选及应用研究”(2017516002) |
| 摘 要： | 对超高压保鲜过程中茶树菇的包装袋分离到的菌株WM1003进行鉴定及系统发育分析，以菌株WM1003的基因组为模板扩增获得了该菌株的16S rRNA基因序列，经过序列分析及系统发育分析，对该菌株进行鉴定；PCR扩增结果表明，该菌株的16S rRNA基因的大小约为1600 bp，其GenBank登录号为HM748831；通过在线BLAST比对，结果表明该菌株属于芽孢杆菌属；基于16S rRNA基因序列的系统发育分析结果表明该菌株同已知菌Paenibacillus jamilae CECT 5266的相似性最高，为99.5%，初步将该分离菌株WM1003鉴定为Paenibacillus sp.；本文通过16S rRNA序列及系统发育分析，对菌株WM1003进行了鉴定，为茶树菇的超高压保鲜技术提供了理论支持。 |
| 关 键 词： | 多样化 多样化 |
| 收稿时间： | 2011/1/19 0:00:00 |
| 修稿时间： | 2011/2/27 0:00:00 |
Identification and Phylogenetic Analysis of a Paenibacillus Strain from the Package of Agrocybe cylindracea Processed by Super High Pressure Technology |
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| Xu Feng,Liu Yu,Zhao Shuang,Wang Hexiang,Zhang Guoqing,Wang Shouxian,Yah Yuelong,Hou Yu,Feng Shanshan.Identification and Phylogenetic Analysis of a Paenibacillus Strain from the Package of Agrocybe cylindracea Processed by Super High Pressure Technology[J].Chinese Agricultural Science Bulletin,2011,27(19):186-192. | |
| Authors: | Xu Feng Liu Yu Zhao Shuang Wang Hexiang Zhang Guoqing Wang Shouxian Yah Yuelong Hou Yu Feng Shanshan |
| Institution: | Xu Feng 1,Liu Yu 1,Zhao Shuang 1,Wang Hexiang 2,Zhang Guoqing 3,Wang Shouxian 1,Yan Yuelong 2,Hou Yu 2,Feng Shanshan 2 ( 1 Institute of Plant and Environmental Protection,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,2 State Key Laboratory for Agribiotechnology,China Agricultural University,Beijing 100193,3 Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture,College of Biotechnology / Beijing University of Agriculture,Beijing 102206) |
| Abstract: | The aim of this study is to identify the bacteria strain numbered as WM1003 isolated from the package of the Agrocybe cylindracea processed by super high pressure technology. The genomic DNA of the strain WM1003 was isolated and purified. 16S rRNA gene sequence was amplified using genomic DNA of the strain WM1003 as template and was sequenced. Phylogenetic position of the WM1003 was analyzed by using MEGA4.0 based on the 16S rRNA sequences. PCR product of the strain WM1003 was about 1600 bp. The 16S rRNA ge... |
| Keywords: | Agrocybe cylindracea Paenibacillus 16S rRNA identification phylogenetic analysis |
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