| 含BADH和Bar基因的植物表达载体的构建 |
| |
| 引用本文： | 李虹章,朱丹华,黄英运,郁晓敏,董德坤.含BADH和Bar基因的植物表达载体的构建[J].中国农学通报,2011,27(18):218-222. |
| |
| 作者姓名： | 李虹章 朱丹华 黄英运 郁晓敏 董德坤 |
| |
| 作者单位： | 1. 浙江师范大学,浙江金华321004;浙江省农业科学院作核所,杭州310021
2. 浙江省农业科学院作核所,杭州,310021 |
| |
| 基金项目： | 转基因生物新品种培育重大专项“转基因抗逆大豆种质创新与新品种培育”(2009ZX08004-006B) |
| |
| 摘 要： | 采用PCR技术扩增出甜菜碱醛脱氢酶基因BADH，并于上下游引物5’端分别添加Xhol和SacI酶切位点和保护性碱基序列；TA克隆PCR产物至pTA2并进行测序验证；双酶切经过测序验证的重组质粒pTA2-BADH和植物表达载体pBA002，分别回收目的基因和pBA002载体片段，并进行连接转化，提取阳性质粒，然后进行双酶切、PCR扩增和进一步的测序验证。结果表明BADH基因已被完整、正确的插入到pBA002载体中，成功构建了含BADH和Bar基因的植物表达载体，为进一步的植物遗传转化奠定了基础。
|
| 关 键 词： | 白点鲑 白点鲑 耗氧率 窒息点 |
| 收稿时间： | 4/6/2011 12:00:00 AM |
| 修稿时间： | 2011/4/26 0:00:00 |
Construction of a Plant Expression Vector Containing the BADH and Bar Genes |
| |
| Li Hongzhang,Zhu Danhua,Huang Yingyun,Yu Xiaomin,Dong Dekun.Construction of a Plant Expression Vector Containing the BADH and Bar Genes[J].Chinese Agricultural Science Bulletin,2011,27(18):218-222. |
| |
| Authors: | Li Hongzhang Zhu Danhua Huang Yingyun Yu Xiaomin Dong Dekun |
| |
| Institution: | Li Hongzhang 1,2,Zhu Danhua 2,Huang Yingyun 1,Yu Xiaomin 2,Dong Dekun 2 (1 Zhejiang Normal University,Jinhua Zhejiang 321004,2 Institute of Corp and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021) |
| |
| Abstract: | BADH (betaine-aldyhyde dehydrogenase,EC 1.2.1.8) cDNA was amplified by PCR,including XhoI (upstream) and SacI (downstream) restriction sites respectively.This BADH fragment was cloned into the vector pTA2 (obtaining pTA2-BADH) and verified by sequencing.Then the fragment was cloned from recombinant plasmid pTA2-BADH into the vector pBA002 by digestion and ligation.The obtained pBA002-BADH vector was further verified by enzyme digestion,PCR amplification,and sequencing.Using the plant expression vector pBA00... |
| |
| Keywords: | BADH plant expression vector Bar |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
