| 产油酵母菌的筛选及其摇瓶发酵条件的优化 |
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| 引用本文： | 杨冉,孙军德.产油酵母菌的筛选及其摇瓶发酵条件的优化[J].农产品加工．学刊,2010(7):66-69. |
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| 作者姓名： | 杨冉 孙军德 |
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| 作者单位： | 沈阳农业大学,辽宁,沈阳,110161 |
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| 摘 要： | 从云杉树枝中筛选出1株高产油脂酵母菌Y-3,通过单因素试验对该菌发酵培养基的碳源、氮源、无机盐和培养条件(接种量、摇床转速、pH值、温度、时间等)进行了研究。结果表明,用酵母菌Y-3生产油脂的最适碳源和氮源分别为葡萄糖和硫酸铵,最适培养条件为:接种量为20%,初始pH值为6,培养温度为28℃,转速为160r/min,培养时间为6d。
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| 关 键 词： | 产油酵母 生物量 油脂含量 发酵条件优化 |
Screening of Oleaginous Yeast and Optimization of Shaking Flask Fermentation Conditions |
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| Yang Ran,Sun Junde.Screening of Oleaginous Yeast and Optimization of Shaking Flask Fermentation Conditions[J].Nongchanpin Jlagong.Xuekan,2010(7):66-69. |
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| Authors: | Yang Ran Sun Junde |
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| Abstract: | A yeast named Y-3 with high oil-producing capacity was screened from spruce.The research was done on the respect of C-source,N-source and cultivation conditions by single factors experiment using the biomass,the content of microbial oils.The optimal fermentation conditions were decided as follows:the optimal carbon and nitrogen source for Y-3 to produce microbial oil were glucose and (NH4)2SO4,the optimal culture conditions at that the amount of inoculation was 20%,initial pH was 6.0,fermentation temperature was 28 ℃,rotational speed was 160 r/min and culture time was 6 d. |
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| Keywords: | oleaginous yeasts biomass content of microbial oils optimization of fermentation conditions |
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