| 火龙果溃疡病病原菌鉴定及生物学特性 |
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| 引用本文： | 易润华,甘罗军,晏冬华,吴泽菁,童依婷,吴凤发.火龙果溃疡病病原菌鉴定及生物学特性[J].植物保护学报,2013,40(2):102-108. |
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| 作者姓名： | 易润华 甘罗军 晏冬华 吴泽菁 童依婷 吴凤发 |
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| 作者单位： | 广东海洋大学农学院生物技术系,湛江,524088 |
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| 基金项目： | 广东省科技厅农业科技攻关项目(2008B020500007) |
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| 摘 要： | 为确定火龙果溃疡病病原菌,应用柯赫氏法则测定了从病样中分离的纯培养物的致病性,根据形态学特性和ITS序列分析对病原菌进行鉴定,并采用菌落生长法和玻片法研究了其生物学特性.结果表明,火龙果溃疡病的病原菌为新暗色柱节孢Neoscytalidium dimidiatum (Penz.) Crous & Slippers.碳氮源对病原菌菌丝生长和产孢量的影响差异明显,菌丝生长和产孢的最佳碳源为蔗糖,菌丝生长最佳氮源为酵母膏,产孢最佳氮源为甘氨酸.温度、pH对病原菌菌丝生长、产孢和孢子萌发的影响差异显著,菌丝生长、产孢、孢子萌发的最佳温度均为30℃；菌丝生长最佳pH为8,产孢和孢子萌发最佳pH为7.光照对病原菌菌丝生长和产孢有抑制作用,对孢子萌发没有明显影响.相对湿度高有利于孢子萌发,高于98％时,萌发率达100％.
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| 关 键 词： | 火龙果 溃疡病 新暗色柱节孢 生物学特性 |
| 收稿时间： | 2012/12/1 0:00:00 |
Identification and biological characteristics of Neoscytalidium dimidiatum causing pitaya canker |
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| Yi Runhu,Gan Luojun,Yan Donghu,Wu Zejing,Tong Yiting and Wu Fengfa.Identification and biological characteristics of Neoscytalidium dimidiatum causing pitaya canker[J].Acta Phytophylacica Sinica,2013,40(2):102-108. |
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| Authors: | Yi Runhu Gan Luojun Yan Donghu Wu Zejing Tong Yiting and Wu Fengfa |
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| Institution: | Department of biotechnology, Agricultural College, Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China;Department of biotechnology, Agricultural College, Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China;Department of biotechnology, Agricultural College, Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China;Department of biotechnology, Agricultural College, Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China;Department of biotechnology, Agricultural College, Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China;Department of biotechnology, Agricultural College, Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China |
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| Abstract: | |
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| Keywords: | pitaya canker disease Neoscytalidium dimidiatum biological characteristic |
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