| 具有杀蚜活性的海洋真菌筛选及其活性成分分析 |
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| 引用本文： | 陈福龙,王秀芳,陈丹,王新伟,任广伟.具有杀蚜活性的海洋真菌筛选及其活性成分分析[J].植物保护学报,2013,40(2):155-159. |
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| 作者姓名： | 陈福龙 王秀芳 陈丹 王新伟 任广伟 |
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| 作者单位： | 中国农业科学院烟草研究所,农业部烟草类作物质量控制重点实验室,青岛266101 |
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| 基金项目： | 农业部烟草类作物质量控制重点开放实验室开放课题(201003),贵州省烟草公司项目(201117) |
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| 摘 要： | 为筛选可产生杀虫活性代谢物的海洋真菌,评价其在农林害虫防治中的潜在应用价值,从21株海洋真菌中复筛得到对烟蚜有较高生物活性的菌株mfz19,用点滴法测定该菌株对烟蚜的触杀作用,并对其发酵液用乙酸乙酯萃取蒸馏和大孔吸附树脂分离纯化后进行GC-MS检测.菌株mfz19发酵液的乙酸乙酯提取物浓度为500 μL/mL时对烟蚜毒杀作用显著,24h校正死亡率为92.91％,LC50为91.40 μL/mL.杀虫活性提取物为一种混合物,其中1,3-二烯丙基脲的相对含量达到90.40％.研究表明该菌株具有较高的杀蚜活性和开发潜力.
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| 关 键 词： | 海洋真菌 次生代谢物 烟蚜 生物测定 |
| 收稿时间： | 2012/12/4 0:00:00 |
Screening of marine fungi with bioactivity against Myzus persicae and analysis of its active components |
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| Chen Fulong,Wang Xiufang,Chen Dan,Wang Xinwei and Ren Guangwei.Screening of marine fungi with bioactivity against Myzus persicae and analysis of its active components[J].Acta Phytophylacica Sinica,2013,40(2):155-159. |
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| Authors: | Chen Fulong Wang Xiufang Chen Dan Wang Xinwei and Ren Guangwei |
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| Institution: | Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong Province, China;Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong Province, China;Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong Province, China;Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong Province, China;Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong Province, China |
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| Abstract: | |
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| Keywords: | marine fungi secondary metabolites Myzus persicae bioassay |
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