| 胡杨组培及愈伤组织增殖研究 |
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| 引用本文： | 张靖,马彦军,贺隆基,张成君.胡杨组培及愈伤组织增殖研究[J].甘肃林业科技,2020(1):17-20. |
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| 作者姓名： | 张靖 马彦军 贺隆基 张成君 |
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| 作者单位： | 甘肃农业大学林学院;甘肃农业大学食品科学与工程学院;甘肃农业大学草业学院 |
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| 基金项目： | 甘肃农业大学省级大学生创新创业训练计划项目资助(201810733037)。 |
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| 摘 要： | 胡杨是我国西北荒漠珍贵先锋树种,但扦插繁殖难以生根。本文以胡杨的枝段为外植体,通过筛选最佳消毒方式和优化培养基,建立胡杨愈伤组织的诱导和增值的培养体系。结果显示:胡杨外植体最佳灭菌法为0.1% HgCl2处理6 min;愈伤组织最佳诱导培养基MS+0.50 mg/L 6-BA+0.10 mg/L NAA,诱导率>80%;愈伤组织适宜增殖培养基MS+0.05 mg/L 6-BA+0.05 mg/L NAA,增殖程度最好。
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| 关 键 词： | 胡杨 组培 愈伤组织诱导 增殖培养 |
Study on Tissue Culture and Callus Proliferation of Populus euphratica |
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| ZHANG Jing,MA Yanjun,HE Longji,ZHANG Chengjun.Study on Tissue Culture and Callus Proliferation of Populus euphratica[J].Journal of Gansu Forestry Science and Technology,2020(1):17-20. |
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| Authors: | ZHANG Jing MA Yanjun HE Longji ZHANG Chengjun |
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| Institution: | (College of Forestry,Gansu Agriculture University,Lanzhou 730070,China;College of Food Science and Engineering,Gansu Agriculture University,Lanzhou 730070,China;College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,China) |
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| Abstract: | Populus euphratica is a valuable forest resource in the desert of northwest China. However, it is difficult for Populus euphratica to take root in normal reproduction. In this paper, the branches of P. euphratica were considered as explants material, through selecting the best sterilization method and optimizing the medium to establish a culture system for induction and proliferation of callus of P. euphratica. The results showed that the optimum sterilization method for the branches of P. euphratica was 0.1% HgCl2 for 6 minutes. The optimum medium for callus induction of the branches of P. euphratica was MS+0.50 mg/L 6-BA +0.10 mg/L NAA, and induction rate was over 80%. The optimum medium for callus proliferation of P. euphratica was MS+0.05 mg/L 6-BA +0.05 mg/L NAA, and has the best degree of proliferation. |
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| Keywords: | populous euphratica tissue culture callus induction proliferation culture |
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