| 猕猴桃品种(系)溃疡病抗性鉴定及不同评价指标的相关性分析 |
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| 引用本文： | 宋雅林,林苗苗,钟云鹏,陈锦永,齐秀娟,孙雷明,方金豹.猕猴桃品种(系)溃疡病抗性鉴定及不同评价指标的相关性分析[J].果树学报,2020(6):900-908. |
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| 作者姓名： | 宋雅林 林苗苗 钟云鹏 陈锦永 齐秀娟 孙雷明 方金豹 |
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| 作者单位： | 中国农业科学院郑州果树研究所 |
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| 基金项目： | 国家自然科学基金青年基金(31701778);国家重点研发计划(2019YFD1000200);河南省科技攻关计划(182102110411);河南省现代农业产业技术体系(S2014-11);国家园艺种质郑州猕猴桃分库(NHGRC2020-NH00-4);河南省财政林木种质资源项目。 |
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| 摘 要： | 【目的】分析不同猕猴桃品种(系)对细菌性溃疡病的抗性以及不同评价指标间的相关性,对鉴定猕猴桃种质资源的抗溃疡病能力和进一步开展抗病相关育种具有非常重要的意义。【方法】利用收集的溃疡病病原菌Pseudomonas syringae pv.Actinidiae(Psa)],经牛肉膏蛋白胨培养基培养,通过猕猴桃离体枝条接种方法和观察接种后的浸染情况,分析了不同猕猴桃品种(系)的抗病性以及病情指数和病斑长度之间的相关性;并用流式细胞仪检测供试材料的倍性,分析抗病性与倍性关系。【结果】不同品种(系)对溃疡病的抗病性存在显著差异,在29份猕猴桃种质资源中‘,华特’等3份毛花猕猴桃材料表现为高抗;‘徐香‘’金魁’等15份种质资源表现为抗病;‘金桃‘’金艳’等4份种质资源表现为耐病;‘Hort16-A’等5份材料表现为感病;中华猕猴桃‘红阳’和‘2-72’表现为高感。不同品种(系)的病情指数与病斑长度呈极显著正相关,相关系数r为0.957。此外,发现在中华猕猴桃中,高感品种‘红阳’‘2-72’和感病品种‘1-74’‘Hort16-A’是二倍体,耐病品种‘金桃’和‘金艳’是四倍体。【结论】抗性评价结果与生产实际发病情况基本相符,说明猕猴桃离体枝条接种方法能够有效的对猕猴桃品种抗病性进行评价,病斑长度可作为重要的评价指标;同一类型品种(系)染色体组倍性越大,抗病性越强。
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| 关 键 词： | 猕猴桃 溃疡病 品种(系) 抗性 相关性 |
Evaluation of resistance of kiwifruit varieties(line)against bacterial canker disease and correlation analysis among evaluation indexes |
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| SONG Yalin,LIN Miaomiao,ZHONG Yunpeng,CHEN Jinyong,QI Xiujuan,SUN Leiming,FANG Jinbao.Evaluation of resistance of kiwifruit varieties(line)against bacterial canker disease and correlation analysis among evaluation indexes[J].Journal of Fruit Science,2020(6):900-908. |
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| Authors: | SONG Yalin LIN Miaomiao ZHONG Yunpeng CHEN Jinyong QI Xiujuan SUN Leiming FANG Jinbao |
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| Institution: | (Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,Henan,China) |
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| Abstract: | 【Objective】In recent years,as the continuous increase of the global kiwifruit cultivation,the occurrance of the bacterial canker disease has been increased The disease has the characteristics of strong pathogenicity,fast transmission speed,and difficulty to eradicate,resulting in restriction of the development of kiwifruit industry.The disease is caused by Pseudomonas syringae pv.actinidiae(Psa).Up to now,the research on the disease mostly focuses on the biological characteristics of the pathogen,cultivation management technology and field control methods.Selection of varieties resistant to the disease would be beneficial to the industry.The analysis of the resistance of different kiwifruit accessions to bacterial canker disease and the correlation between different evaluation indexes are of great significance to breeding new kiwifruit varieties resistant to the disease.【Methods】The kiwifruit varieties(lines)used in this study were all from the Kiwifruit Germplasm Collection of Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences.Pseudomonas syringae pv.actinidiae(Psa),kiwifruit bacterial canker pathogen,was provided by the Institute of Horticulture,Zhejiang Academy of Agricultural Sciences.Firstly,specific primers PsaF1 and PsaR1 were used for PCR amplification to identify the correctness of the strain,and then the Psa was cultured in beef extract peptone medium(shaker temperature 22-24℃,oscillation frequency 200 r·min^-1,time 24 h),and the microbial concentration of the Psa was diluted to 1×108 colony-forming units cfu·mL^-1 prior to inoculation.The One-year old detached shoots,approximately 0.8 cm in diameter,were sterilized with 75%alcohol and then cut into 10 cm shoots,the ends of the shoots were dipped in candle wax to reduce dehydration.A wound of about 1-1.5 cm was made with a file from each end of the shoot and the Psa was added to the wound with a pipette.The inoculated shoots were placed in trays,which were placed in an artificial climate incubator at 22-24℃and 80%relative humidity for 12 h day/night cycles.The ploidy of different kiwifruit varieties(lines).was detected by flow cytometry to analyze the relationship between disease resistance and ploidy.The test data were statistically analyzed using Excel and SPSS 13.0 software.【Results】6 days after the Psa inoculation of kiwifruit isolated shoots,the disease infection occurred in some varieties(lines).The inoculation area became reddish brown and exhibited milky white bacterial pus.21 days after the Psa inoculation,the disease indexes of different varieties were calculated and classified according to the evaluation standard of kiwifruit bacterial canker disease resistance.The results indicated that‘Hongyang’and‘2-72’exhibited high susceptibility;‘Hort16-A’‘6-65’‘1-74’‘14-57’and‘6-20’exhibited susceptibility;‘2-43’‘Jintao’‘Jinyan’‘Youxi-52’showed tolerance;‘Xuxiang’‘Hward’‘Bulunuo’‘Hongbaoshixing’‘SE1-5’‘P1-2’‘P4-2’‘N4-32’‘P6-1’‘P3-3’‘11-17’‘N4-25’‘N14-9’‘Jinkui’and‘TML’showed resistance;and‘N12-16’‘N12-3’and‘Huate’showed high resistance.In addition,21 days after the Psa inoculation,lesion length was measured with a vernier caliper.The average length of disease spots of high susceptible varieties,susceptible varieties,tolerant varieties,resistant varieties and high resistant varieties were 29.53,15.63,10.12,2.86,0.79 mm,respectively.The results showed that the higher the disease resistance of kiwifruits was,the smaller the length of lesion was;The SPSS correlation analysis showed that the disease indexes of 29 kiwifruit varieties(lines)were significantly positively correlated with the lesion lengths,and the correlation coefficient R was 0.957.In addition,the ploidy of 29 kiwifruit varieties(lines)was detected by flow cytometry.Among A.chinensis,high susceptible varieties‘Hongyang’,‘2-72’and susceptible varieties‘1-74’and‘Hort16A’were diploid,while tolerance varieties‘Jintao’and‘Jinyan’were tetraploid.The nine A.deliciosa kiwifruit materials were all hexaploid ploidy,among them three materials showed susceptibility,one showed tolerance,and the remaining 5 showed resistance,which accounted for 55.6%.The seven A.arguta kiwifruit materials were all tetraploid,and all showed resistantce.The six A.eriantha kiwifruit materials were all diploid,and all showed resistance or high resistantce.There was a tendency that among A.chinensis,the biger the ploidy number was,the stronger the disease resistance degree was.However,the effect of interspecific ploidy on resistance was not significant.【Conclusion】Among the methods for the identifying the resistance of kiwifruit to the canker disease,kiwifruit detached shoot inoculation was a common and effective method.The mothod was simple to operate and easy to master,and the identification results were statistically reliable.The disease index was significantly and positively correlated with the lesion length.It seems that the tetraploidy of the kiwifruit accessions would be more resistant to the disease than the diploidy ones in A.chinensis. |
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| Keywords: | Kiwifruit Canker disease Cultivars(lines) Resistance Correlation |
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