Biodegradation of carbofuran by <Emphasis Type="Italic">Pichia anomala</Emphasis> strain HQ-C-01 and its application for bioremediation of contaminated soils |
| |
| Authors: | Liu Yang Shaohua Chen Meiying Hu Weining Hao Peng Geng Yanbo Zhang |
| |
| Institution: | (1) Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, 510642, People’s Republic of China; |
| |
| Abstract: | A novel yeast named HQ-C-01 was isolated from activated sludge and identified as Pichia anomala based on the morphology and 18S rDNA sequence analysis. The HQ-C-01 strain degraded 95.2% of carbofuran when the insecticide
was used as the only C source and added at 50 mg/L in a mineral salts medium within 48 h. The optimal concentration, temperature,
and pH of medium for degradation of carbofuran were 50 mg/L, 30°C, and pH 7.5, respectively. Strain HQ-C-01 could also effectively
degrade other carbamate insecticides including carbaryl, indoxacarb, and fenobucarb, and the degradation rates were 99%, 85%,
and 67%, respectively. Gas chromatography–mass spectrometry analysis showed that the strain metabolized carbofuran to produce
benzofuranol as the intermediate metabolite, which was further degraded. Degradation of carbofuran added at 50 mg/kg of soil
was higher in yeast-inoculated soil than in the control. These results indicated that strain HQ-C-01 may potentially be used
in bioremediation of carbofuran-contaminated soil. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
