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不同基质对铁皮石斛组培苗成活和生长的影响
引用本文:肖昌泰,毛昆明,杨自琼,闫培睿,周莹.不同基质对铁皮石斛组培苗成活和生长的影响[J].现代农业科技,2012(4):147-149.
作者姓名:肖昌泰  毛昆明  杨自琼  闫培睿  周莹
作者单位:云南农业大学资源与环境学院,云南昆明,650201
基金项目:云南省科技厅项目“石斛种苗扩繁技术”
摘    要:试验设3种基质处理,即刨花∶细锯末=1∶6(处理1)、桤木树皮∶细锯末=5∶1(处理2)、杉木树皮∶细锯末=5∶1(处理3),比较铁皮石斛的成活率、茎粗、株高、根长、根数、叶片数等,筛选适合铁皮石斛生长的最佳基质。该试验条件下,移栽30 d后铁皮石斛组培苗成活率在处理1中为91.3%,在处理2中为97.5%,在处理3中为95.5%;移栽60 d后,其株高、根长在处理1中为6.40、3.26 cm,在处理2中为7.40、5.25 cm,在处理3中为6.45、5.10 cm;其根数在处理1、2、3分别为5.0、6.5、5.6条,茎粗则分别为0.255、0.582、0.542 cm,对应的叶片数分别为9.0、14.5、13.0片。研究初步显示,青木树皮∶细锯末=5∶1是用于铁皮石斛试管苗移栽的最佳基质。

关 键 词:基质  铁皮石斛  成活率  生长

Effects of Different Substrates on the Survival and Growth of the Plantlet of Dendrobium officinale
Authors:O Chang-tai  O Kun-ming  G Zi-qiong  N Pei-rui  HOU Ying
Institution:(Department of Resources and Environment,Yunnan Agricultural University,Kunming Yunnan 650201)
Abstract:With wood shavings∶fine sawdust=1∶6(treatment 1)、Alder bark∶fine sawdust=5∶1(treatment 2) and Fir bark∶fine sawdust=5∶1(treatment 3)for different treatments,compared the survival rate of Dendrobium officinale,stem diameter,plant height,root length,root number,leaf number,etc.Under the experimental conditions,30 days after transplanting survival rate of Dendrobium officinale tissue culture was 91.3%(sreatment 1),97.5%(treatment 2) and 95.5%(treatment 3).60 days after transplanting,the plant height,root length in the treatment 1 was 6.40 cm and 3.26 cm,respectively,in the treatment 2 was 7.40 cm and 5.25 cm,respectively,in the treament 3 was 6.45 cm and 5.10 cm,respectively.The root was number in the treatment 1,2,3 was 5.0,6.5 and 5.6,respectively.Stem diameter was 0.255 cm,0.582 cm and 0.542 cm respectively,the number of leaves was 9.0 and 14.5,13.0,respectively.Preliminary study showed that alder bark∶fine sawdust=5∶1was the best substrate for Dendrobium officinale plantlets transplanted.
Keywords:stroma  Dendrobium officinale  survival  growth
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