| 鹅源H5N1禽流感病毒感染对雏鸭红细胞免疫功能的影响 |
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| 引用本文： | 郑世民,葛依阳,马宏伟,高雪丽,刘超男,吕晓萍.鹅源H5N1禽流感病毒感染对雏鸭红细胞免疫功能的影响[J].东北农业大学学报,2019,50(1):45-51. |
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| 作者姓名： | 郑世民 葛依阳 马宏伟 高雪丽 刘超男 吕晓萍 |
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| 作者单位： | 东北农业大学动物医学学院, 哈尔滨 150030;黑龙江省实验动物与比较医学重点实验室, 哈尔滨 150030;东北农业大学动物医学学院, 哈尔滨 150030;黑龙江省实验动物与比较医学重点实验室, 哈尔滨 150030;东北农业大学动物医学学院, 哈尔滨 150030;黑龙江省实验动物与比较医学重点实验室, 哈尔滨 150030;东北农业大学动物医学学院, 哈尔滨 150030;黑龙江省实验动物与比较医学重点实验室, 哈尔滨 150030;东北农业大学动物医学学院, 哈尔滨 150030;黑龙江省实验动物与比较医学重点实验室, 哈尔滨 150030;东北农业大学动物医学学院, 哈尔滨 150030;黑龙江省实验动物与比较医学重点实验室, 哈尔滨 150030 |
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| 摘 要： | 将100只21日龄雏鸭随机分为AIV感染组(I)和对照组(C),应用免疫学和细胞培养及MTT等方法动态检测AIV感染雏鸭血液红细胞免疫黏附能力、红细胞免疫自身调控能力以及红细胞促外周血淋巴细胞增殖能力。结果表明,雏鸭感染AIV后1～14 d,红细胞C_(3b)受体花环(E-CRR)率明显低于对照组,而红细胞免疫复合物花环(E-ICR)率明显高于对照组;AIV感染雏鸭后3～21 d,红细胞C_(3b)受体花环促进率(RFER)显著低于对照组,且红细胞C_(3b)受体花环抑制(RFIR)率显著高于对照组;AIV感染雏鸭的红细胞对血液T、B淋巴细胞增殖促进能力明显低于对照组。表明AIV感染引起的雏鸭红细胞免疫黏附功能、自身调控能力和T、B淋巴细胞增殖能力下降,与AIV感染导致雏鸭外周血免疫功能降低密切相关。
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| 关 键 词： | 禽流感病毒 雏鸭 红细胞免疫 免疫黏附 |
Effect of goose source H5N1 avian influenza virus infection to erythrocyte immune function of duckling |
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| Institution: | ,School of Veterinary Medicine,Northeast Agricultural University,Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine |
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| Abstract: | In this study, one hundred 21-day-old ducklings were randomly assigned into two groups: AIV infection group(I) and un-infection group(C). Erythrocyte immune adherence function,erythrocyte immune self-regulation ability and regulative ability of erythrocyte acting on lymphocyte proliferation were performed to figure out the mechanism of duckling immune pathogenesis with MTT method. The results showed that erythrocyte C_(3b) receptor rosette(E-CRR)ratio of duckling infected with AIV was significantly decreased than that AIV un-infection ducklings in 1-14 d. erythrocyte immune complex rosette(E-ICR) ratio of AIV infection group was significantly higher than that of control group in1-14 d; the immune enhancing factor of AIV infection ducklings were lower than that in control group from 3-21 d, while the immune inhibition factor of AIV infection group were higher from 3-21 d than that in control group; Besides, the ability of T, B lymphocytes proliferation in the AIV infection group was obviously lower than that in control group. Therefore, this study illustrated that the AIV infectioninduced declines of erythrocyte immune adherence function, self-regulation ability and T, B lymphocyte proliferation ability in ducklings are closely related to erythrocyte immune. |
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| Keywords: | AIV duckling erythrocyte immune immune adherence |
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