| 甘蓝型油菜全基因组DELLA蛋白基因家族的鉴定和表达分析 |
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| 引用本文： | 贾永鹏,李开祥,昝领兄,姚艳梅,杜德志.甘蓝型油菜全基因组DELLA蛋白基因家族的鉴定和表达分析[J].中国油料作物学报,2019,41(3):360. |
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| 作者姓名： | 贾永鹏 李开祥 昝领兄 姚艳梅 杜德志 |
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| 作者单位： | 青海大学／青海省农林科学院／青海省春油菜遗传改良重点实验室／国家油菜改良中心青海分中心 ／农业部春油菜科学观测实验站／青海省春油菜研究开发中心，青海西宁，８１００００ |
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| 基金项目： | 现代农业产业技术体系建设专项资金资助（CARS-12）；青海省春油菜遗传改良重点实验室 (2017-ZJ-Y09)；青海省重大专项：青海省高原特色农作物现代种业创新体系建设（2017-NK-A7）；青海省自然科学基金项目（2019-ZJ-972Q） |
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| 摘 要： | DELLA蛋白是参与赤霉素调控途径的关键因子，对调节植物的生长发育具有重要的作用。为了揭示甘蓝型油菜中DELLA蛋白家族的分布和特征，利用生物信息分析了家族成员、序列特征、进化关系和组织表达。结果表明，甘蓝型油菜中有13个DELLA蛋白基因，被定位到8条染色体和2条随机序列（chrC09_random和chrCnn_random）上；系统进化树分析发现，该家族被聚为4个亚家族，第一亚家族含有7个基因，第二、三和四亚家族各包括2个基因； DELLA基因呈组织特异性表达，其中BnaA10g17240D、BnaC09g40420D、BnaCnng68300D和BnaC05g47760D在各组织中表达都相对较高，在茎中BnaA06g34810D、BnaA09g18700D和BnaC09g52270D表达最高。本研究为后续基因的功能研究提供参考。
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| 关 键 词： | 甘蓝型油菜 DELLA基因家族 基因表达 |
Genome-wide identification and expression of DELLA protein gene family in Brassica napus |
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| JIA Yong-peng,LI Kai-xiang,ZAN Ling-xiong,Yao Yan-mei,DU De-zhi.Genome-wide identification and expression of DELLA protein gene family in Brassica napus[J].Chinese Journal of Oil Crop Sciences,2019,41(3):360. |
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| Authors: | JIA Yong-peng LI Kai-xiang ZAN Ling-xiong Yao Yan-mei DU De-zhi |
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| Institution: | Qinghai University; Qinghai Academy of Agriculture and Forestry Sciences; Key Laboratory of Spring Rape Genetic Improvement Qinghai Province; Qinghai Sub-center of National Rape Improvement Center; Spring rapeseed scientific observation experimental station of the Ministry of Agriculture; Qinghai Province Spring Rapeseed Research and Development Center, Xining, 810016, China |
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| Abstract: | DELLA protein is a key factor involved in GA regulatory pathways and plays an important role in regulating plant growth and development. In order to reveal the distribution and characteristics of DELLA protein family in Brassica napus, family members, sequence characteristics, evolutionary relationships and tissue expression were analyzed using bioinformatics. The results showed that there were 13 DELLA protein genes in Brassica napus, located in 8 chromosomes with 2 random sequences (chrC09_random and chrCnn_random); The phylogenetic tree analysis found that the family was clustered into 4 subfamilies. One subfamily contains 7 genes, and the second, third and fourth subfamilies each included 2 genes. The expression analysis of different tissues showed tissue-specific expression in each tissue, and BnaA10g17240D, BnaC09g40420D、BnaCnng68300D and BnaC05g47760D were relatively high in each tissue. Among the stems, BnaA06g34810D、BnaA09g18700D and BnaC09g52270D had the highest expression. This study provided a reference for studying the genes functionality in the future. |
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| Keywords: | Brassica napus L DELLA gene family gene expression |
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