| STM和CUC2基因对不同抗寒性白菜型冬油菜生长点的调控研究 |
| |
| 引用本文： | 赵玉红,马 骊,常 瑜,白 静,蒲媛媛,牛早霞,金姣姣,柴昊,朵茂惠,刘丽君,武军艳,方 彦,李学才,孙万仓.STM和CUC2基因对不同抗寒性白菜型冬油菜生长点的调控研究[J].中国油料作物学报,2019,41(2):157-165. |
| |
| 作者姓名： | 赵玉红 马 骊 常 瑜 白 静 蒲媛媛 牛早霞 金姣姣 柴昊 朵茂惠 刘丽君 武军艳 方 彦 李学才 孙万仓 |
| |
| 作者单位： | 甘肃农业大学农学院，甘肃省油菜工程技术研究中心，甘肃省干旱生境作物学重点实验室，
甘肃省作物遗传改良与种质创新重点实验室，甘肃兰州，７３００７０ |
| |
| 基金项目： | 国家现代农业产业技术体系(CARS-12)；国家自然科学基金(31460356)；973计划（2015CB150206）；863计划（2016YFD0101300）；甘肃省重大专项(17ZD2NA016-4)；甘肃省现代农业产业技术体系(GARS-TSZ-1) |
| |
| 摘 要： | 为研究抗寒性强的白菜型冬油菜(Brassica rapa)调控生长点(shoot apical meristem,SAM)凹陷生长的SHOOT MERISTEMLESS(STM)和CUP-SHAPED COTYLEDON 2(CUC2)基因,采用RT-PCR技术克隆两基因并进行了表达分析。STM基因CDS序列开放阅读框长度为1 554bp,编码517个氨基酸,该基因在进化上高度保守,其序列属于PLN03226超家族。CUC2基因开放阅读框长度为1 104bp,编码367个氨基酸,该基因保守序列属于NAM超家族,是一个主要由不规则卷曲与延伸链组成的亲水性蛋白。荧光定量分析表明,相同温度处理下,STM基因在抗寒性较强、生长点凹陷的白菜型冬油菜陇油6号和陇油7号中相对表达量均低于抗寒性较弱、生长点凸起的天油4号和Lenox,而CUC2基因相对表达量的变化趋势与STM基因相反;低温(0℃、4℃和-4℃)处理72h后,白菜型冬油菜生长点中STM、CUC2基因均上调表达,表明STM和CUC2基因可能参与抗寒应答及白菜型冬油菜生长点对低温胁迫的响应。
|
| 关 键 词： | 白菜型油菜 冬油菜 凹陷的生长点 克隆 低温胁迫 表达分析 |
Regulation of STM and CUC2 genes on apical meristem of cold-resistant winter Brassica rapa |
| |
| ZHAO Yu-hong,MA Li,CHANG Yu,BAI Jing,PU Yuan-yuan,NIU Zao-xia,JIN Jiao-jiao,CHAI Hao,DUO Mao-hui,LIU Li-Jun,WU Jun-yan,FANG Yan,LI Xue-cai,SUN Wan-cang.Regulation of STM and CUC2 genes on apical meristem of cold-resistant winter Brassica rapa[J].Chinese Journal of Oil Crop Sciences,2019,41(2):157-165. |
| |
| Authors: | ZHAO Yu-hong MA Li CHANG Yu BAI Jing PU Yuan-yuan NIU Zao-xia JIN Jiao-jiao CHAI Hao DUO Mao-hui LIU Li-Jun WU Jun-yan FANG Yan LI Xue-cai SUN Wan-cang |
| |
| Institution: | College of Agronomy, Gansu Agricultural University, Rapeseed Engineering Research Center of Gansu Province, Key Laboratory of Arid Land Crop Science in Gansu Province, Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou 730070, China |
| |
| Abstract: | To understand the molecular regulation of SHOOT MERISTEMLESS (STM) and CUP-SHAPED COTYLEDON 2 (CUC2) genes on shoot apical meristem growth, both genes were cloned from winter Brassica rapa by RT-PCR. CDS sequence of STM gene was obtained to be of 1 554 bp in length, encoding 517 amino acids, which was highly evolutionally conserved and belonged to PLN03226 superfamily. CUC2 open reading frame was 1 104 bp long, encoding 367 amino acids, belonging to NAM superfamily. CUC2 was predicted to be a hydrophilic protein mainly composed of irregular curly and extended chains. Fluorescence quantitative analysis showed that under the same temperature, the relative expression of STM gene in winter B. rapa (cvs Longyou 6 and Longyou 7 with strong cold resistances, which had recessed growth cones) were lower than that with raised cone in Tianyou 4 and Lenox. But the relative expression of CUC2 gene was the opposite. After 72 h of cold (0oC, 4 oC and -4 oC)) treatment, STM and CUC2 genes in the growth cones were up-regulated, indicating that both genes were involved in cold response in the growth cone to low temperature. |
| |
| Keywords: | Brassica rapa winter type recessed growth cone cold stress gene expression |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《中国油料作物学报》浏览原始摘要信息 |
| 点击此处可从《中国油料作物学报》下载免费的PDF全文 |
|
