| 根癌农杆菌法转化康乃馨的条件探索 |
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| 引用本文： | 丁小维,刘开辉,邓百万,陈文强,刘飞虎.根癌农杆菌法转化康乃馨的条件探索[J].安徽农业科学,2008,36(13):5329-5330. |
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| 作者姓名： | 丁小维 刘开辉 邓百万 陈文强 刘飞虎 |
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| 作者单位： | 1. 陕西理工学院,陕西省资源生物重点实验室,陕西汉中,723001
2. 云南大学生命科学学院,云南昆明,650091 |
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| 摘 要： | 目的]为构建高效的康乃馨遗传转化体系奠定基础。方法]以康乃馨叶片为受体材料,通过抗G418筛选,探讨预培养时间、根癌农杆菌菌液浓度、共培养时间等因素对根癌农杆菌介导康乃馨遗传转化的影响。结果]20 mg/ml G418是康乃馨叶片转化的适宜浓度。随着预培养时间的增加,康乃馨叶片的存活率降低,预培养2~3 d时抗性芽分化率较高。当根癌农杆菌OD600为0.5~0.8时,抗性芽分化率较高,适合康乃馨转化。共培养2~3 d时,康乃馨叶片生长状态好,抗性芽分化率最高。结论]预培养、共培养各2~3 d,菌液浓度OD600为0.5~0.8是根癌农杆菌介导康乃馨遗传转化的适宜条件。
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| 关 键 词： | 康乃馨 根癌农杆菌 遗传转化 |
Exploration on the Genetic Transformation Conditions of Dianthus caryophyllus L.Mediated by Agrobacterium tumefaciens |
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| DING Xiao-wei et al.Exploration on the Genetic Transformation Conditions of Dianthus caryophyllus L.Mediated by Agrobacterium tumefaciens[J].Journal of Anhui Agricultural Sciences,2008,36(13):5329-5330. |
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| Authors: | DING Xiao-wei |
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| Abstract: | Objective] The research aimed to lay the foundation for constructing the highly-efficient genetic transformation system of Dianthus caryophyllus L.Method] With the leaves of D.caryophyllus as receptor materials,the effects of such factors as preculture time,the concn.of Agrobacterium tumefaciens and coculture time on the genetic transformation of D.caryophyllus mediated by A.tumefaciens were studied through G418 screening. Result] 20 mg/ml G418 was the suitable concn.for the transformation of D. caryophyllus leaves.With the increasing of preculture time,the survival rate of D.caryophyllus leaves were reduced.When preculture time was 2～3 d,the differentiation rate of resistant buds were higher.When OD600 of A.tumefaciens was 0.5～0.8,the differentiation rate of resistant buds were higher and favorable for the transformation of D.caryophyllus.When coculture time was 2～3 d,the growth state of D.caryophyllus leaves were good and the differentiation rate of resistant buds were highest.Conclusion] Both precultue and coculture for 2～3 d and the bacterial liquid concn.OD600 of 0.5～0.8 were the suitable conditions for the genetic transformation of D.caryophyllus mediated by A.tumefaciens. |
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| Keywords: | Dianthus caryophyllus L Agrobacterium tumefaciens Genetic transformation |
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