Induce triploidy by heat shock in Eurasian perch,Perca fluviatilis |
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| Institution: | 1. Department of Forestry and Natural Resources, Purdue University, 195 Marsteller St., West Lafayette, IN 47907, USA;2. U.S. Geological Survey, Great Lakes Science Center, 1451 Green Road, Ann Arbor, MI 48105, USA;3. Illinois-Indiana Sea Grant, Purdue University, 195 Marsteller St., West Lafayette, IN 47907, USA;1. University of Manitoba, 50 Sifton Rd., Winnipeg, MB R3T 2N2, Canada;2. Fisheries and Oceans Canada, 501 University Crescent, Winnipeg, MB R3T 2N6, Canada;3. University of Victoria, 3800 Finnerty Rd, Victoria, BC V8P 5C2, Canada;4. Fisheries and Oceans Canada, Suite 301 5204 50th Ave., Yellowknife, NT X1A 1E2, Canada |
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| Abstract: | In Eurasian perch (Perca fluviatilis), females grow significantly faster than males. Moreover, gonadal development has a significant negative impact on somatic growth and fillet yield. In order to induce sterility, triploidy induction was attempted by subjecting fertilised eggs to heat shocks. Different combinations of temperature (28, 30, 34, 35 and 36 °C), duration (2, 5, 10 and 25 min) and time of shock initiation (TI = 3, 5 and 7 min post-fertilisation) were tested. Flow cytometry analysis was used to assess ploidy level of control and heat-shocked larvae. Low intensity (28–30 °C) and long duration (10 and 25 min) shocks lead to significantly higher survival (44 ± 26%) and triploidisation (71 ± 26%) rates than high intensity (34–36 °C) and short duration (2 and 5 min) shocks (17 ± 19% and 21 ± 26%, respectively). The most effective conditions for efficient triploidy induction were low intensity shock of 30 °C, applied 5 min post-fertilisation for 25 min. This treatment led to the production of all-triploid populations (100%) with up to 43% survival rate. |
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