| 高效液相色谱法测定柚叶中柚皮苷的含量 |
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| 引用本文： | 蓝松.高效液相色谱法测定柚叶中柚皮苷的含量[J].安徽农业科学,2007,35(33):10581-10582. |
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| 作者姓名： | 蓝松 |
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| 作者单位： | 宜春学院生物工程研究所,江西宜春,336000 |
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| 摘 要： | 建立柚叶中柚皮苷含量的高效液相色谱分析方法。采用Kromasil C18色谱柱,流动相为甲醇∶水∶醋酸(35∶61∶4),流速为1.0ml/min,检测波长为283 nm。柚皮苷进样量在0.101~2.202μg线性良好,平均回收率101.27%,RSD为1.41%,所测样品的柚皮苷含量0.84%~1.25%,且嫩叶柚皮苷含量稍高于老叶。该法简便、快速、准确,可用于柚叶中柚皮苷的含量测定。
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| 关 键 词： | 柚皮苷 柚叶 含量测定 |
| 文章编号： | 0517-6611(2007)33-10581-01 |
| 修稿时间： | 2007年6月21日 |
Determination of Naringin in Leaves of Citrus grandis(L.) Osbeck by the Method of HPLC |
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| LAN Song.Determination of Naringin in Leaves of Citrus grandis(L.) Osbeck by the Method of HPLC[J].Journal of Anhui Agricultural Sciences,2007,35(33):10581-10582. |
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| Authors: | LAN Song |
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| Abstract: | To establish a method for the determination of naringin in leaf of Citrus grandis(L.)Osbeck by HPLC,the chromatographic condions were as follows:Kromasil C18 column with methel-water-acetic acid(35∶61∶4) as mobile phase,the detection wavelength at 283 nm and the flow rate being 1.0 ml/min.The results indicated a linearity of naringin in the range of the average recovery was 101.27%(RSD=1.41).The conclusion was the method was rapid and precise,and could be used to determine naringin in leaves of Citrus grandis(L.)Osbeck. |
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| Keywords: | Naringin Citrus grandis(L )Osbeck Analysis |
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