| 成年兔睾丸支持细胞的分离纯化研究 |
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| 引用本文： | 夏凡,李青旺,韩增胜,陈晓宇,龙玲.成年兔睾丸支持细胞的分离纯化研究[J].家畜生态,2005(5). |
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| 作者姓名： | 夏凡 李青旺 韩增胜 陈晓宇 龙玲 |
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| 作者单位： | 西北农林科技大学动物科技学院，西北农林科技大学动物科技学院，西北农林科技大学动物科技学院，西北农林科技大学动物科技学院，西北农林科技大学动物科技学院 陕西 杨凌 712100，陕西 杨凌 712100 燕山大学环境与化学工程学院，河北 秦皇岛 066004，陕西 杨凌 712100，陕西 杨凌 712100，陕西 杨凌 712100 |
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| 摘 要： | 本试验以5月龄公兔为试验动物,去除睾丸被膜及血管,分离出完整的曲精细管后 用0．5％胰蛋白酶和0．1％胶原酶消化并进行低渗处理,将制成的细胞悬液植入25 cm2的培 养瓶,于38．7℃、5％CO2条件下培养,结果表明:经1:3Hanks超纯水低渗处理后,离体支持 细胞纯度和活率分别达到72．04％和84．5％,显著提高了离体支持细胞的获取量和活率。
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| 关 键 词： | 兔 支持细胞 分离纯化 培养 |
Isolation and Purification of Testis Sertoli Cells in Adult Rabbit |
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| XIA Fan,LI Qing-wang,HAN Zeng-sheng,CHEN Xiao-yu,LONG Ling.Isolation and Purification of Testis Sertoli Cells in Adult Rabbit[J].Ecology of Domestic Animal,2005(5). |
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| Authors: | XIA Fan LI Qing-wang HAN Zeng-sheng CHEN Xiao-yu LONG Ling |
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| Abstract: | Decapsulated and removed tests from 5-mo-old rabbit, then the whole seminiferous tubules were dispersed. The subsequent enzymatic digestion was used with 0. 5% trypsin and 0. 1% collagenase, the resulting tissues were made into cell suspension and subjected to hypotonic shock, after that, the Sertoli cells were cultured at 38. 7 C with 5% CO2 in 25 cm2 flasks. The result showed that, after treated with hypotonic shock in 1/3 group, the purity and viability of isolated Sertoli cells reached to 72. 04% and 84. 5% respectively. It increased the quality and viability of isolated Sertoli cells greatly. |
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| Keywords: | rabbit sertoli cell isolation cultivation |
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