| 细胞外Ca2+和K8对鲤鱼脑垂体离体生长激素分泌的影响 |
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| 引用本文： | 林信伟,林浩然.细胞外Ca2+和K8对鲤鱼脑垂体离体生长激素分泌的影响[J].水产学报,1993,17(1):07-13. |
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| 作者姓名： | 林信伟 林浩然 |
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| 作者单位： | 中山大学生物系，中山大学生物系 广州 510275，广州 510275 |
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| 基金项目： | 高等学校博士点科学基金 |
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| 摘 要： | 采用脑垂体离体灌流孵育系统,研究细胞外 Ca~(2+)和 K~+对鲤鱼脑垂体基础的和鲑鱼促性腺激素释放激素(sGnRH)刺激的生长激素(GH)分泌的影响。离体灌流孵育的鲤鱼脑垂体基础 GH分泌和 sGnRH 刺激的 GH 分泌都是细胞外 Ca~(2+)依赖的,缺细胞外 Ca~(2+)存在时,基础 GR分泌显著下降,2分钟脉冲式 sGnRH 刺激的 GH 分泌反应接近消失。Ca~(2+)通道阻滞剂异搏定以剂量依存形式显著抑制基础的和2分钟脉冲式sGnRH 刺激的 GH 分泌,表明细胞外 Ca~(2+)的作用至少部分通过细胞膜电位敏感性 Ca~(2+)通道。50mM K~+显著刺激基础GH 分泌,并显著加强高剂量sGnRH 刺激的GH 分泌,且K~+的作用是细胞外 Ca~(2+)依赖的。
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| 关 键 词： | 生长激素 鲤鱼 |
| 收稿时间： | 7/1/1992 12:00:00 AM |
| 修稿时间： | 1992/10/1 0:00:00 |
EFFECTS OF EXTRACELLULAR CALCIUM AND POTASSIUM ON GROWTH HORMONE SECRETION FROM COMMON CARP PITUITARY IN VITRO |
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| Lin Xinwei and Lin Haoran.EFFECTS OF EXTRACELLULAR CALCIUM AND POTASSIUM ON GROWTH HORMONE SECRETION FROM COMMON CARP PITUITARY IN VITRO[J].Journal of Fisheries of China,1993,17(1):07-13. |
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| Authors: | Lin Xinwei and Lin Haoran |
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| Institution: | Lin Xinwei,Lin Haoran Department of Biology,Zhongshan University,Guangzhou 510275 |
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| Abstract: | The effects of extracellular Ca~(2+) and K~+ on the basal and salmonGnRH (sGnRH)-stimulated growth hormone (GH) release from pituitary of com-mon carp were studied using an in vitro perifusion system for pituitary fragmen-ts. The basal and sGnRH-stimulated GR secretion from erifused pituitary ofcommon carp were extracellular Ca~(2+)-dependent. In the absence of extracellularCa~(2+), the significant reduction in the basal GH secretion and almost disappearan-ce in the GH release responses to 2--min pulses of sGnRH (1, 10 and 100nM)were observed. Voltage-sensitive calcium channels (VSCC) antagonist verapam il(VER) significantly inhibited the basal GH release and CH release responses to2- min pulses of sGnRH (1,10 and 100nM) in a dose--dependent manner, indica-ting that the effects of extracellular Ca~(2+) on the basal and sGnRH-stimulated GHrelease are, at least partially, via the action of VSCC. 50mM K~+ significantlystimulated the basal GR release from perifused pituitary of common carp, andmarkedly potentiated the action of pulses of high dose of sGnRH (100nM) on GHsecretion. The effects of elevated K~+ were extracellular Ca~(2+)--dependent. |
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| Keywords: | salmon GnRH growth hormone calcium potassium in vitro perifusion common carp |
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