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| rbcS启动子的克隆及活性鉴定 | |
| 引用本文： | 王旺田,张金文,刘玲玲,陈正华.rbcS启动子的克隆及活性鉴定[J].甘肃农业大学学报,2004,39(3):255-260. |
| 作者姓名： | 王旺田 张金文 刘玲玲 陈正华 |
| 作者单位： | 1. 甘肃省作物改良与种质创新重点实验室,甘肃农业大学,甘肃,兰州,730070 2. 甘肃省作物改良与种质创新重点实验室,甘肃农业大学,甘肃,兰州,730070;中国科学院西北高原生物研究所,青海,西宁,810080;甘肃亚盛集团博士科研工作站,甘肃,兰州,730035 3. 甘肃亚盛集团博士科研工作站,甘肃,兰州,730035 |
| 基金项目： | 甘肃省扶贫办资助项目(编号:) |
| 摘 要： | 利用PCR技术从豌豆(Pisum satium Linn)基因组DNA中分离了rbcS基因启动子及叶绿体定位信号编码序列。序列分析表明,扩增片段(1256bp)与已报道序列的相应区域同源性达98.41 %。将其与绿色荧光蛋白基因(GFP)串连在一起,构建了植物表达载体,通过基因枪介导法转化烟草叶片及洋葱表皮细胞。结果表明这一表达系统增强了绿色荧光蛋白基因的瞬时表达,从而为外源基因在转基因植物中的高效表达提供了新的工具,可以实现目的基因的组织特异性和光诱导性表达。 |
| 关 键 词： | rbcS 基因启动子 绿色荧光蛋白基因 植物表达载体 活性鉴定 |
| 文章编号： | 1003-4315(2004)03-0255-06 |
Isolated the promoter of rbcS and determination of its activity |
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| WANG Wang-tian,ZHANG Jin-wen,,LIU Ling-ling,CHEN Zheng-hua.Isolated the promoter of rbcS and determination of its activity[J].Journal of Gansu Agricultural University,2004,39(3):255-260. | |
| Authors: | WANG Wang-tian ZHANG Jin-wen LIU Ling-ling CHEN Zheng-hua |
| Institution: | WANG Wang-tian1,ZHANG Jin-wen1,2,3,LIU Ling-ling1,CHEN Zheng-hua3 |
| Abstract: | The upstream regulatory region of the small subunit of ribulose-1, 5-bisposphate carboxylase gene and chloroplast the code array of orients signal were separated from pea genomic DNA via PCR. Array analysis indiacated that the amplification and reporting array had 98.41 % of homology sequences. Plant expression vector was constructed by ligating this promoter and transport peptide with green fluorescent protein (GFP) gene then transferred into tobacco leaf and epidermis cell of onion by microprojectile bombardment. The result showed that this expression system strengthened the instantaneous expression of green fluorescence protein. A new tool was offered for foreign genes?high expression in the transgenic plant, and it could achieve tissue-special and light-regulated expression of interest gene. |
| Keywords: | rbcS gene promoter green fluorescent protein (GFP) transient expression vector determination of activity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |