| 几株鸡新城疫病毒的分离鉴定及其基因型分析 |
| |
| 引用本文： | 孙丰廷,郑杰,张发明,崔惠娟,杨国良,张红,张坦,张亮,陈玲,李静,王文泉.几株鸡新城疫病毒的分离鉴定及其基因型分析[J].中国畜牧兽医,2012,39(10):76-79. |
| |
| 作者姓名： | 孙丰廷 郑杰 张发明 崔惠娟 杨国良 张红 张坦 张亮 陈玲 李静 王文泉 |
| |
| 作者单位： | 1. 北京华都诗华生物制品有限公司,北京 102600;2. 云南红河州动物疫病预防控制中心, 云南红河 661100 |
| |
| 摘 要： | 从几个发生禽病的地区分离到6株病毒,经过血凝试验、血凝抑制试验、中和试验、分子生物学试验等确定为鸡新城疫病毒;根据GenBank公布的新城疫F基因强弱毒株相关基因序列设计合成1对特异性引物,扩增出的F基因片段长度约为610 bp,测序后进行序列比对,并分析其核苷酸序列和氨基酸序列,结果显示,有3株具有新城疫强毒株特性,同时还具备新城疫Ⅶ基因型特征,另外根据平均死亡时间(MDT)、脑内致病指数(ICPI)和静脉致病指数(IVPI)指标测定结果,最终判定这3株是新城疫强毒株且属于基因Ⅶ型,其余3株病毒分离株与La Sota的核酸序列同源性与氨基酸序列同源性均为99%。
|
| 关 键 词： | 新城疫 F基因 强毒株 基因Ⅶ型 |
| 收稿时间： | 2012-03-21 |
Isolation,Identification and Genotypic Analysis of Several Strains of Newcastle Disease Virus |
| |
| SUN Feng-ting,ZHENG Jie,ZHANG Fa-ming,CUI Hui-juan,YANG Guo-liang,ZHANG Hong,ZHANG Tan,ZHANG Liang,CHEN Ling,LI Jing,WANG Wen-quan.Isolation,Identification and Genotypic Analysis of Several Strains of Newcastle Disease Virus[J].China Animal Husbandry & Veterinary Medicine,2012,39(10):76-79. |
| |
| Authors: | SUN Feng-ting ZHENG Jie ZHANG Fa-ming CUI Hui-juan YANG Guo-liang ZHANG Hong ZHANG Tan ZHANG Liang CHEN Ling LI Jing WANG Wen-quan |
| |
| Institution: | 1. Beijing CEVA Huadu Biological Products Co.,Ltd., Beijing 102600, China;2. Yunnan Honghe Animal Disease Prevention and Control Center, Honghe 661100, China |
| |
| Abstract: | Six strains of virus isolated from several areas of poultry occurred Newcastle disease, through HA,HI,neutralization test, and molecular biology experiments were identified as Newcastle disease virus. According to F gene sequences published in GenBank, a pair of specific primers was designed and synthesized, length of approximately 610 bp was amplified, alignment and analysis of nucleotide and amino acid sequences were conducted. The results showed that there were 3 lines with virulent Newcastle disease virus strain characteristics, also had the Newcastle Ⅶ genotype features. According to MDT, ICPI and IVPI index determination result, we finally determined that the 3 strains were virulent strains of NDV and belonged to Ⅶ genotype. The homology of nucleic acid and amino acid sequence between remaining 3 strains of virus and La Sota were both 99%. |
| |
| Keywords: | ND F gene virulent strain genotype Ⅶ |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
