| 试纸条法与琼脂扩散法检测IBDV抗体的比较(英文) |
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| 引用本文： | 张进良,张文通,胡思顺,毕丁仁,王喜亮,肖运才.试纸条法与琼脂扩散法检测IBDV抗体的比较(英文)[J].农业科学与技术,2012(9):1826-1828. |
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| 作者姓名： | 张进良 张文通 胡思顺 毕丁仁 王喜亮 肖运才 |
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| 作者单位： | 华中农业大学动物医学院;商丘师范学院生命科学学院;山东省农业科学院滨州分院;山东省滨州畜牧兽医研究院 |
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| 摘 要： | 目的]比较分析IBDV抗体的试纸条与琼脂糖扩散两种检测方法。方法]用自行研制的试纸条法检测鸡血清中的鸡传染性法氏囊病毒抗体,并与传统的琼脂扩散法检测结果进行比较。结果]试纸条较琼脂凝胶扩散试验敏感8倍,在216份临床样品测定试验中,检出率更高,同时其又具有特异性强、易保存、操作简单快速等优点,故试纸条更适合于临床抗体监测。结论]该研究结果表明试纸条可替代现有的血清学方法,在抗体监测方面具有极大的推广应用价值。
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| 关 键 词： | 传染性法氏囊病 琼脂扩散法 试纸条 |
Comparative Studies on Detection of Antibodies against Infectious Bursal Disease Virus with Test Strips and Agar Gel Immunodiffusion Method |
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| Jinliang ZHANG,Wentong ZHANG,Sishun HU,Dingren BI, Xiliang WANG,Yuncai XIAO.Comparative Studies on Detection of Antibodies against Infectious Bursal Disease Virus with Test Strips and Agar Gel Immunodiffusion Method[J].Agricultural Science ＆ Technology,2012(9):1826-1828. |
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| Authors: | Jinliang ZHANG Wentong ZHANG Sishun HU Dingren BI Xiliang WANG Yuncai XIAO |
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| Institution: | 1. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; 2. College of Life Science, Shangqiu Normal University, Shangqiu 476000, China; 3. Shandong Binzhou Animal Science & Veterinary Medicine Academy, Shandong Academy of Agricultural Sciences, Binzhou 256600, China |
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| Abstract: | Objective] This study aimed to compare the detection results of antibodies against infectious bursal disease virus with test strips and agar gel immunodiffusion method.Method] Antibodies against infectious bursal disease virus in chicken serum were detected using test strips developed in our laboratory,and the results were compared with that using traditional agar diffusion method.Result] The comparative study of the two methods showed that the sensitivity of test strips was eight times over agar gel immunodiffusion; test strips showed higher detection rate in the determination test of 216 clinical samples,with high specificity,easy preservation,and simple and rapid operation,thereby being more suitable for the monitoring of clinical antibodies.Conclusion] Test strips could replace the existing serological methods,having great promotion and application value in antibody monitoring. |
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| Keywords: | Infectious bursal disease Agar gel immunodiffusion Test strips |
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