| 一种从马鹿粪便中提取DNA的改进方法(英文) |
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| 引用本文： | 日沙来提·吐尔地,艾斯卡尔·买买提,日孜汗·阿布地艾尼,阿米拉·阿布来提,马合木提·哈力克.一种从马鹿粪便中提取DNA的改进方法(英文)[J].农业科学与技术,2012(4):698-700,900. |
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| 作者姓名： | 日沙来提·吐尔地 艾斯卡尔·买买提 日孜汗·阿布地艾尼 阿米拉·阿布来提 马合木提·哈力克 |
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| 作者单位： | 新疆大学生命科学与技术学院资源生物研究所 |
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| 基金项目： | Supported by the National Natural Science Foundation of China(31060152);the Natural Science Foundation of the Xinjiang Uygur Autonomous Region,China(2010211A02);the Key Program for Animal Sciences of Xinjiang University,China~~ |
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| 摘 要： | 目的]介绍一个从马鹿粪便中提取DNA的改进方法。方法]本实验采用的是,在传统的CTAB裂解法的基础下,根据马鹿粪便的特征摸索出来的改进方法。结果]采用该方法从天山马鹿粪便中提取了高质量的粪便DNA并扩增了天山马鹿线粒体细胞色素b基因片段,通过测序检测,同时提取马鹿肌肉和皮毛样品的DNA作为对照,进一步证实了提取的可靠性。结论]该方法提取过程中不需要用蛋白酶K;所提取的DNA不需要用DNA纯化试剂盒纯化,可直接用PCR扩增,因此,费用量很低。
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| 关 键 词： | 马鹿 非损伤性取样 改进的DNA提取方法 粪便DNA |
An Improved Method for DNA Extraction from the Faeces of Red Deer |
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| Institution: | Turdi RISALAT,Muhammat ASKAR,Abduany RIZIHAN,Ablat AMILA,Halik MAHMUT College of Life Science and Technology,Xinjiang University,Urumqi 830046,China |
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| Abstract: | Objective] To introduce an improved method for DNA extraction from the faeces of red deer.Method] Based on the traditional method of CTAB lysis,we proposed an improved DNA extraction method according to the characteristics of red deer faeces.Result] This improved method extracted high-quality fecal DNA from Tianshan red deer and amplified the mitochondrial cytochrome b gene.With the muscle and fur DNA of red deer as the control,the sequencing results further confirmed the reliability of the method.Conclusion] The method requires no proteinase K in the process of extraction,and the extracted DNA can be used for PCR amplification directly without the purification of DNA purification kit,thus,it is cost-saving. |
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| Keywords: | Red deer Noninvasive sampling Improved method for DNA extraction Faecal DNA |
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