| 降解蒽遗传重组菌在蒽污染环境中的降解和存活能力研究 |
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| 引用本文： | 尹红梅,刘慧知,张甲耀.降解蒽遗传重组菌在蒽污染环境中的降解和存活能力研究[J].农业环境科学学报,2007,26(2):544-547. |
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| 作者姓名： | 尹红梅 刘慧知 张甲耀 |
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| 作者单位： | 1. 湖南省微生物研究所,湖南,长沙,410009
2. 武汉大学环境科学系,湖北,武汉,430072 |
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| 摘 要： | 为了提高外源蒽降解菌An降解蒽的能力和存活能力，采用原生质体电融合技术，在蒽降解菌An和土生优势菌Tu之间实行了原生质体电融合，并从再生的融合子F中筛选到具有特定性状的后代菌株。把融合子接种于蒽污染的土壤与水环境中，研究其降解蒽的能力与存活能力。结果表明，在蒽污染的土壤中，40d时，融合子F与亲本An对蒽的降解率分别为70．7％、55．5％，融合子比出发菌株高15．2％；100h内融合子F和亲本An对水环境中蒽的降解转化率分别为75％、67．2％。说明在蒽污染的土壤和水环境中．在初始接种量相同的情况下，融合子的存活率明显高于出发菌株。
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| 关 键 词： | 原生质体电融合 降解能力 存活能力 生物强化 |
| 文章编号： | 1672-2043(2007)02-0544-04 |
| 修稿时间： | 2006-12-22 |
Degradation and Survival of Genetic Recombination Microorganisms in Anthracene-contaminated Soil and Water |
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| YIN Hong-mei,LIU Hui-zhi,ZHANG Jia-yao.Degradation and Survival of Genetic Recombination Microorganisms in Anthracene-contaminated Soil and Water[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2007,26(2):544-547. |
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| Authors: | YIN Hong-mei LIU Hui-zhi ZHANG Jia-yao |
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| Institution: | 1. Hunan Institute of Microbiology, Changsha 410009, China; 2. Department of Environment Science, Wuhan University, Wuhan 430072, China |
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| Abstract: | In order to improve the ability of degrading anthracene and the survival of exogenous bacteria An, protoplast electrofusion was performed between An which can degrade anthracene effectively and Tu which has high survival ability, and then a fusant with specific properties was selected from the regenerated colonies. The degradation performance on anthracene and survival of fusant which was inoculated in soil and water were tested. The results showed that, in soil, after 40 days degradation, the removal rates of anthracene were 70.7% and 55.5% by fusant and An, respectively. And the fusant degrading rate increased by 15.2%. In water environment, after 100 hours reaction, the removal rates were 75% and 67.2% by fusant and An, respectively. So the fusants had much higher population density than the original strains in the same amount of inoculation in anthracene-contaminated soil and water environment. |
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| Keywords: | protoplast electrofusion degradation survival bioaugmentation |
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