| 荧光相图法研究溶菌酶在盐酸胍溶液中的变性 |
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| 引用本文： | 李向荣,晁淑军.荧光相图法研究溶菌酶在盐酸胍溶液中的变性[J].安徽农业科学,2010,38(22):11679-11680. |
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| 作者姓名： | 李向荣 晁淑军 |
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| 作者单位： | 新乡医学院化学教研室,河南新乡,453003;新乡医学院化学教研室,河南新乡,453003 |
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| 摘 要： | 采用荧光相图法分析盐酸胍诱导溶菌酶变性的荧光数据,建立溶菌酶在盐酸胍溶液中失活的变性模型。结果表明,盐酸胍浓度从0.5mol/L变化至3.0mol/L时,溶菌酶从天然态转变为部分折叠中间态,盐酸胍浓度从3.0mol/L变化至6.0mol/L时,溶菌酶从中间态转变为去折叠态。说明盐酸胍诱导溶菌酶变性的过程符合典型的＂三态模型＂。
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| 关 键 词： | 溶菌酶 荧光相图法 盐酸胍 变性 |
Research on the Denaturation of Lysozyme in Guanidine Hydrochloride Solution by Fluorescence Phase Diagram Method |
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| LI Xiang-rong et al.Research on the Denaturation of Lysozyme in Guanidine Hydrochloride Solution by Fluorescence Phase Diagram Method[J].Journal of Anhui Agricultural Sciences,2010,38(22):11679-11680. |
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| Authors: | LI Xiang-rong |
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| Institution: | LI Xiang-rong et al (Department of chemistry,Xinxiang Medical College,Xinxiang,Henan 453003) |
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| Abstract: | The fluorescence phase diagram method was used to analyze the fluorescence data of antalzyme denaturation induced with guanidine hydrochloride for establishing the denaturation model of deactivation of lysozyme in guanidine hydrochloride solution.The results indicated that when the guanidine hydrochloride concn.varied from 0.5 mol/L to 3.0 mol/L,the lysozyme was transformed from nature state to be a partiauyfolded intermediate;when the guanidine hydrochloride concn.varied from 3.0 mol/L to 6.0 mol/L,the lys... |
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| Keywords: | Lysozyme Fluorescence phase diagram method Guanidine hydrochloride Denaturation |
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