| Abstract: | The type strain WVU 1853 and field strains SG, N26 and A642 of Mycoplasma synoviae were examined for their requirement for nicotinamide-adenine dinucleotide (NAD) for in vitro growth. All the strains grew and could be repeatedly passaged in Frey broth medium supplemented with filter-sterilised NAD. In modified Frey broth medium from which NAD was omitted and broth medium for which the supplements including yeast extract and NAD were autoclaved, only strains N26 and A642 could be grown and passaged. The growth curves of strain N26 determined in broth media with and without NAD were similar. These results indicated that there are differences in NAD-requirement for in vitro growth among strains of M synoviae. |
