| 表达新城疫病毒HN基因的重组火鸡疱疹病毒的构建 |
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| 引用本文： | 赵军,张秀根,陈溥言,蔡宝祥.表达新城疫病毒HN基因的重组火鸡疱疹病毒的构建[J].畜牧兽医学报,2000,31(2):150-154. |
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| 作者姓名： | 赵军 张秀根 陈溥言 蔡宝祥 |
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| 作者单位： | 南京农业大学动物医学院,南京,210095 |
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| 摘 要： | 应用聚合酶链反应（ＰＣＲ）技术，用设计带有限制性酶切位点的特异地扩增子人起始密码子开始的１．８ｋｂ新城疫病毒（ＮＤＶ）血凝素－神经氨酸（ＨＮ）基因开放式阅读框（ＯＲＦ），然后插入ｐＴＫ２Ｂ的Ｎｂｅ１位点，构建了含ＮＤＶＨＮ基因的插入载体ＰＴＫＨＮ１和ＰＴＫＨＮ２，将其与感染火鸡疱疹病毒（ＨＶＴ）细胞的总ＤＮＡ共转民纤维细胞（ＣＥＦ），经有限稀释法和Ｄｏｔ－ｂｌｏｔ筛选，得到含有ＨＮ基因的重组体ｒＨ
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| 关 键 词： | 重组HVT HN基因 ND基因工程疫苗 病毒载体 禽用 |
CONSTRUCTION OF A RECOMBINANT HERPESVIRUS OF TURKEY EXPRESSING THE HEMAGGLUTININ-NEURAMINIDASE GENE OF NEWCASTLE DISEASE VIRUS |
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| Zhao Jun,Zhang Xiugen,Chen Puyan,Cai Baoxiang.CONSTRUCTION OF A RECOMBINANT HERPESVIRUS OF TURKEY EXPRESSING THE HEMAGGLUTININ-NEURAMINIDASE GENE OF NEWCASTLE DISEASE VIRUS[J].Acta Veterinaria et Zootechnica Sinica,2000,31(2):150-154. |
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| Authors: | Zhao Jun Zhang Xiugen Chen Puyan Cai Baoxiang |
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| Abstract: | WT5BZ] A 1 8kb fragment of Newcastle disease virus (NDV) hemagglutinin neuraminidase(HN) gene was amplified by polymerase chain reaction (PCR) with primers containing enzyme cleavage sites,and inserted into a nonessential gene of herpesvirus of turkey (HVT) Recombinant viruses were screened by Dot blot hybridization with digoxigenin labelled probe and purified by plaque formation technique the expression of NDV HN gene could be detected by Western blot in CEF cells infected with recombinant virus Recombinant HVT strains were replicated stably in cell cultures WT5HZ] |
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| Keywords: | Recombinant HVT NDV HN gene |
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