| 褪黑素对体外培养绵羊垂体细胞分泌FSH和LH的影响 |
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| 引用本文： | 马友记,张勇,李发弟,赵兴绪,柳纪省.褪黑素对体外培养绵羊垂体细胞分泌FSH和LH的影响[J].家畜生态,2005(3). |
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| 作者姓名： | 马友记 张勇 李发弟 赵兴绪 柳纪省 |
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| 作者单位： | 甘肃农业大学动物医学院 甘肃兰州 730070
(马友记，张勇，赵兴绪)，甘肃农业大学动物科技学院甘肃兰州 730070
(李发弟)，中国农业科学院兰州兽医研究所 甘肃兰州 730046(柳纪省) |
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| 基金项目： | 甘肃省自然科学基金项目(ZS001-A21和ZS011-A25)资助。 |
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| 摘 要： | 本文采用体外细胞培养和放射免疫测定法(RIA)的方法,研究了褪黑素(MLT)对季节性繁殖的蒙古母羊垂体细胞分泌促卵泡素(FSH)和促黄体素(LH)的作用。结果表明:当单独用递增的MLT(10、100、1000、2000pg/mL)处理原代垂体细胞时,随时间的延长FSH的分泌量极显著下降(P<0.01),但对LH的基础分泌没有影响;无论用10IU/mLhCG单独刺激,还是用不同剂量MLT与10IU/mLhCG共同刺激垂体细胞,FSH和LH的分泌都极显著高于对照组(P<0.01),但与MLT的剂量没有关系。
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| 关 键 词： | 褪黑素 垂体细胞 促卵泡素 促黄体素 放射免疫测定法 |
Effects of Melatonin on FSH and LH Production by Hypophysis Cells of Ewes in Vitro |
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| MA You-ji,ZHANG Yong,LI Fa-di.Effects of Melatonin on FSH and LH Production by Hypophysis Cells of Ewes in Vitro[J].Ecology of Domestic Animal,2005(3). |
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| Authors: | MA You-ji ZHANG Yong LI Fa-di |
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| Institution: | MA You-ji~1,ZHANG Yong~1,LI Fa-di~ |
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| Abstract: | The effect of melatonin (MLT) on Follicle Stimulation Hormone(FSH) and Luteinizing Hormone(LH)production was investigated in cultured hypophysis cells isolated from breeding ewes. The study adapted the way of cell culture and carried different treatment. The hormone concentration in cell culture media were analyzed by RIA. The results were as follows: When hypophysis cells were treated with increasing dose of MLT (10?100?1000?2000 pg/mL)singly, the concentration of FSH were lower significantly(P<0.01), but no effect on basal LH production. When hypophysis cells were treated with hCG(10 IU/mL)singly or hCG and MLT (10?100?1000?2000 pg/mL), the concentration of FSH and LH would increase, but had no relation with the concentration of MLT. |
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| Keywords: | melatonin hypophysis cells follicle stimulation hormone luteinizing hormone RIA |
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