Measurement of plasma leucine flux in rainbow trout (Salmo gairneri R.) using osmotic pump. Preliminary investigations on influence of diet |
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| Authors: | Benoit Fauconneau Sophie Tesseraud |
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| Institution: | (1) Fish Nutrition Research Unit, Station d'Hydrobiologie, I.N.R.A., St Pée Sur Nivelle, B.P. 3, 64310 Ascain, France;(2) Present address: Fish Physiology Laboratory, I.N.R.A., Campus de Beaulieu, 35042 Rennes Cedex, France;(3) Present address: Poultry Research Station, INRA. C.R.A., Tours-Nouzilly, Monnaie, 37380, France |
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| Abstract: | A method for the direct measurement of plasma amino acid flux, in rainbow trout, using the continuous infusion of L-U-14C]-leucine with ALZET mini-osmotic pumps implanted into the peritoneal cavity, was developed. The fish were fed successively
on three different diets (a commercial control diet, a semi-purified diet and a purified diet) during the 4 weeks of experiment.
The amounts of radioactivity in the free pool and the protein of both the plasma and skin mucus were measured in these fish.
The total flux of leucine was calculated either from the specific activity of leucine in the plasma (61.8 mg leucine. 100
g-1.d-1) or from the amounts of labelled and unlabelled leucine flowing into the skin mucus protein (61.5 mg leucine. 100g-1.day-1). The total plasma flux was not affected by changes in the diet. The contributions of total leucine oxidation and whole body
protein turnover to plasma leucine flux (80% and 20% respectively) were estimated in fish fed the semi-purified diet. |
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| Keywords: | rainbow trout amino acid metabolism protein synthesis amino acid oxidation leucine methods |
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