| 哈夫尼菌降解毒死蜱条件的优化及动力学模型建立 |
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| 引用本文： | 陶玉贵,王其进,谷浩,倪正,秦昌云.哈夫尼菌降解毒死蜱条件的优化及动力学模型建立[J].农业环境保护,2011(3):449-454. |
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| 作者姓名： | 陶玉贵 王其进 谷浩 倪正 秦昌云 |
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| 作者单位： | 安徽工程大学生物与化学工程学院,安徽芜湖241000 |
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| 基金项目： | 安徽省科技攻关计划面上项目（08010302169） |
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| 摘 要： | 利用响应面法优化哈夫尼菌降解毒死蜱的条件,并建立了降解模型。采用Box-Behnken设计试验,以响应面法进行优化,结果表明,哈夫尼菌降解毒死蜱的最优条件为：蔗糖浓度0.31%,毒死蜱初浓度51.33mg·L-1,培养温度30.7℃,在该条件下理论预测毒死蜱降解率可达74.7%。通过假设和验证,得出哈夫尼菌降解毒死蜱为一级动力学模型,哈夫尼菌能水解毒死蜱P-O键,属一级酶促反应。
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| 关 键 词： | 哈夫尼菌 毒死蜱 响应面 动力学 |
Optimization of Hafnia sp.to Chlorpyrifos Degradation Conditions and a Kinetics Model |
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| TAO Yu-gui,WANG Qi-jin,GU Hao,NI Zheng,QIN Chang-yun.Optimization of Hafnia sp.to Chlorpyrifos Degradation Conditions and a Kinetics Model[J].Agro-Environmental Protection,2011(3):449-454. |
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| Authors: | TAO Yu-gui WANG Qi-jin GU Hao NI Zheng QIN Chang-yun |
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| Institution: | (Department of Biochemical Engineering,Anhui Polytechnic University,Wuhu 241000,China) |
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| Abstract: | The objective of this study was to determine the optimum conditions for the biodegradation of chlorpyrifos by Hafnia sp.,and to establish a kinetic model to simulate the degradation process.Response surface methodology（RSM） based on Box-Bohnken experimental design was employed to determine the optimum conditions.Results indicated that the optimum conditions for chlorpyrifos degradation based on response surface and contour plots were as follows：concentration of sucrose,0.31%（w）;initial concentration of chlorpyrifos,51.33 mg·L-1;culture temperature,30.7 ℃.In addition,the predicted value of chlorpyrifos degradation rate under the optimum conditions was 74.7%,which was in good agreement with the experimental result：under the optimum conditions,the degradation rate of chlorpyrifos was 72.2% after 72 hour＇s culture.Moreover,according to hypothetical kinetic model for chlopyrifos degradation,the kinetics for chlorpyrifos degradation could be described by a first-order equation,which is similar to an enzyme-catalyzed reaction.A possible mechanism for the degradation of chlorpyrifos by Hafnia sp was that this microorganism had produced an enzyme that can cleave the P-O bond of chlorpyrifos. |
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| Keywords: | Hafnia sp chlorpyrifos response surface methodology（RSM） kinetics |
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