| 丁草胺和苄嘧磺隆对钝顶螺旋藻的毒性效应研究 |
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| 引用本文： | 贺鸿志,余景,骆世明,李拥军,邱晓辉,罗琼珠,黎华寿.丁草胺和苄嘧磺隆对钝顶螺旋藻的毒性效应研究[J].农业环境保护,2011(6):1070-1075. |
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| 作者姓名： | 贺鸿志 余景 骆世明 李拥军 邱晓辉 罗琼珠 黎华寿 |
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| 作者单位： | [1]华南农业大学农业部生态农业重点开放实验室,广州510642 [2]广东省渔业生态环境重点实验室,广州510300 [3]广东省高等学校农业生态与农村环境重点实验室,广州510642 [4]中山市农产品质量监督检验所,广东中山528403 |
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| 基金项目： | 国家自然科学基金（31000246 30870413）; 广东省渔业生态环境重点实验室开放基金（LFE-2009-07）; 广东省自然科学基金（9451064201003803）; 华南农业大学校长科学基金（2008K022） |
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| 摘 要： | 采用实验室摇瓶实验法研究了除草剂丁草胺和苄嘧磺隆对钝顶螺旋藻的毒性效应。结果表明,丁草胺浓度与藻生长（以OD560计）存在明显的剂量-效应关系,64mg·L^-1丁草胺严重抑制藻生长,比生长速率为0.111d-1,仅为对照的24%。在7d实验时间内EC50值随染毒时间延长呈指数下降,96h的EC50值为45.4mg·L^-1,到第7d降为15.2mg·L^-1。与丁草胺相似,在实验浓度范围内随浓度增加,苄嘧磺隆对钝顶螺旋藻的生长抑制作用逐渐增强,最高浓度44.8mg·L^-1处理的比生长速率为0.458d-1,约为对照的82%。在8d实验时间内EC50值随染毒时间延长呈指数增长,96h的EC50值为51.3mg·L^-1,到第8d达到215mg·L^-1。两种除草剂在较高浓度条件下均使钝顶螺旋藻藻丝变短,部分藻丝由螺旋型变为直线型,随浓度增加直线型藻丝逐渐增加,到最高浓度几乎全部变为直线型,且藻细胞颜色变浅。依据96h-EC50值判断丁草胺对钝顶螺旋藻的毒性大于苄嘧磺隆,二者毒性强度均为中等,但随着染毒时间延长丁草胺的毒性作用增强而苄嘧磺隆的毒性逐渐降低。同时,高浓度的两种除草剂使部分藻丝断裂变短,且诱导藻丝形态由螺旋型向直线型转变。
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| 关 键 词： | 丁草胺 苄嘧磺隆 钝顶螺旋藻 毒性 |
Toxic Effects of Butachlor and Bensulfuron Methyl on Cyanobacterium Spirulina platensis |
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| HE Hong-zhi,YU Jing,LUO Shi-ming,LI Yong-jun,QIU Xiao-hui,LUO Qiong-zhu,LI Hua-shou.Toxic Effects of Butachlor and Bensulfuron Methyl on Cyanobacterium Spirulina platensis[J].Agro-Environmental Protection,2011(6):1070-1075. |
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| Authors: | HE Hong-zhi YU Jing LUO Shi-ming LI Yong-jun QIU Xiao-hui LUO Qiong-zhu LI Hua-shou |
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| Institution: | 1.Key Laboratory of Ecological Agriculture of Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China;2.Key Laboratory of Fishery Ecology and Environment, Guangdong Province, Guangzhou 510300, China;3.Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, Guangzhou 510642, China; 4.Zhongshan Quality Supervision ＆ Inspection Institute of Agricultural Products, Zhongshan 528403, China) |
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| Abstract: | The toxic effects of butachlor and bensulfuron methyl on S. platensis were studied using shaking flash method. It showed that significant dosage effect of butachlor on algal growth（expressed in OD560） existed. The growth of S. platensis exposed under 64 mg·L^-1 butachlor was severely inhibited. Specific growth rate was 0.111 d-1. It was only 24% of the control. During the 7 d experimental period, EC50 decreased exponentially. The EC50 in 96 h was 45.4 mg·L^-1, and it dropped to 15.2 mg·L^-1 in day 7. Like butachlor, dosage effect of bensulfuron methyl on growth inhabitation on S. platensis was also significant. Specific growth rate under 44.8 mg·L^-1 treatment was 0.458 d-1. It was 82% of the control. During the 8 d experimental period, EC50 value increased exponentially. EC50 in 96 h was 51.3 mg·L^-1, and EC50 in day 8 was 215 mg·L^-1. When treated with these two herbicides, algal filaments of S. platensis became shorter, and some of the spiraled filaments changed into a linear form. Percentage of linear algal filaments increased while herbicides concentration increased. Nearly all algal filaments changed into linear form with much lighter color in the algal culture with the highest concentration of herbicides. Although toxicity of the two herbicides to S. platensis was moderate, butachlor exhibited higher toxicity than bensulfuron methyl to the alga according to 96 h-EC50. With longer exposure time, toxicity of butachlor increased while that of bensulfuron methyl decreased gradually. Lots of spiraled filaments of S. platensis switched to linear ones under high concentration treatments, and some filaments even disconnected and became much shorter. |
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| Keywords: | butachlor bensulfuron methyl Spirulina platensis toxicity |
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