| 土霉素在鸡粪中的残留及降解规律研究 |
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| 引用本文： | 王志强,韩艳,张斌.土霉素在鸡粪中的残留及降解规律研究[J].农业环境保护,2011(10):2124-2129. |
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| 作者姓名： | 王志强 韩艳 张斌 |
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| 作者单位： | [1]扬州大学兽医学院,江苏扬州225009 [2]扬州市畜牧兽医站,江苏扬州225009 |
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| 基金项目： | 江苏省高校自然科学基础研究项目（08KJB230003）; 江苏省“六大人才高峰”资助项目 |
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| 摘 要： | 检测了健康肉鸡以不同剂量土霉素混饲给药后,在不同时间鸡粪中土霉素的含量变化,并比较避光及人工光照条件下灭菌鸡粪中土霉素的降解规律,以及在避光条件下灭菌、未灭菌及灭菌后加降解菌处理,鸡粪中土霉素的降解规律。结果表明,健康肉鸡混饲给予土霉素（100、200、400mg·kg-1）后,鸡粪中土霉素排泄量在给药后6～8h达到高峰,峰浓度分别为13.58、36.15mg·kg-1和50.73mg·kg-1,并于30、62h和72h时检测不到土霉素;对鸡粪中土霉素的浓度随时间变化趋势进行曲线拟合,发现各处理组的这种变化趋势均符合一级动力学方程Ct=C0e-kt。经灭菌鸡粪中土霉素的降解受光照条件和土霉素初始浓度的影响,初始浓度为10、20、40mg·kg-1时,避光条件下,土霉素降解半衰期分别为55.5、123.8d和173.3d;人工光照条件下,鸡粪中的土霉素降解较快,降解半衰期分别为14.8、21.1d和27.5d。此外,无论是在避光还是人工光照条件下,较高初始浓度组的土霉素降解速率均比较低初始浓度组慢。去除光照因素后,鸡粪中所含土霉素的降解主要受微生物及土霉素初始浓度的影响,初始浓度为10、20、40mg·kg-1时,灭菌组土霉素降解半衰期分别为78.7、103.4d和157.5d,未灭菌组为40.5、50.6d和97.6d,灭菌后加降解菌组为14.5、33.6d和51.3d,说明鸡粪中微生物的存在可加快土霉素的降解。相同处理条件下,土霉素的降解速率随药物初始浓度的升高而降低。
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| 关 键 词： | 鸡粪 土霉素 HPLC 降解 |
Residue and Degradation of Oxytetracycline in Chicken Feces |
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| WANG Zhi-qiang,HAN Yan,ZHANG Bin.Residue and Degradation of Oxytetracycline in Chicken Feces[J].Agro-Environmental Protection,2011(10):2124-2129. |
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| Authors: | WANG Zhi-qiang HAN Yan ZHANG Bin |
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| Institution: | 1.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;2.Yangzhou Animal Husbandry and Veterinary Station,Yangzhou 225009,China) |
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| Abstract: | In the first part of the study,the concentration of oxytetracycline in broiler chicken feces was examined after administration of different oral dosages of oxytetracycline by HPLC（high performance liquid chromatography）.In the second part of the study,the degradability of oxytetracycline in sterilized chicken feces was tested under photophobic or illumination condition.In the third part of the study,the degradability of oxytetracycline in chicken feces was tested under different microbial conditions without illumination.The results were as follows.After feed medication in healthy broiler chicken,oxytetracycline was excreted in feces as the parent compound.When the dosage of feed medication was 100 mg·kg-1,200 mg·kg-1 and 400 mg·kg-1 feed,the concentration of oxytetracycline in chicken feces reached the peak at the 6th～8th hour after administration,with a peak concentration of 13.58 mg·kg-1,36.15 mg·kg-1 and 50.73 mg·kg-1 respectively.Oxytetracycline was undetectable at the 30th,62nd and 72nd hour after administration,respectively.The degradation process of oxytetracycline in chicken feces could be evaluated on the basis of the first order kinetics equation as Ct=C0 e-kt.The degradation of oxytetracycline in sterilized chicken feces was affected by illumination and the initial concentration of oxytetracycline.When the initial concentration of oxytetracycline was 10 mg·kg-1,20 mg·kg-1 and 40 mg·kg-1,the half-life of oxytetracycline in sterilized chicken feces was 55.5 d,123.8 d and 173.3 d respectively under photophobic condition and was 14.8 d,21.1 d and 27.5 d under illumination condition.Under photophobic condition,the degradability of oxytetracycline in chicken feces was affected by microbes and the initial concentration of oxytetracycline.When the initial concentration of oxytetracycline was 10 mg·kg-1,20 mg·kg-1 and 40 mg·kg-1,the half-life of oxytetracycline in chicken feces was 78.7 d,103.4 d and 157.5 d respectively with sterilized treatment,and was 40.5 d,50.6 d and 97.6 d respectively with unsterilized treatment,and was 14.5 d,33.6 d and 51.3 d respectively with added degrading bacteria treatment.Furthermore,in the second and third part of the study,the rate of oxytetracycline degradation increased when its initial concentration decreased under the same condition. |
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| Keywords: | chicken feces oxytetracycline HPLC degradation |
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