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| HPLC法检测水牛血浆中吡喹酮的研究 | |
| 引用本文： | 孙雨,卜仕金.HPLC法检测水牛血浆中吡喹酮的研究[J].中国兽药杂志,2010,44(3):9-12. |
| 作者姓名： | 孙雨 卜仕金 |
| 作者单位： | 1. 扬州大学兽医学院,江苏扬州,225009 2. 安徽省巢湖市居巢区散兵镇人民政府,安徽巢湖,238052 3. 中国农业科学院家禽研究所,江苏扬州,225009 |
| 基金项目： | 中国农业科学院上海兽医研究所资助项目 |
| 摘 要： | 建立了一种简便、灵敏、可重复的高效液相色谱法检测水牛血浆中吡喹酮(Praziquantel,PZQ)的含量。吡喹酮在C18(250 mm×4.0 mm,5μm)柱中可以很好地与杂质峰分离开。其保留时间、紫外检测波长、流动相、流速、色谱柱温度分别为10.6 min、220 nm、甲醇∶水(70∶30,V/V)、0.8 mL/min和40℃。水牛血浆样品(0.5 mL)经乙酸乙酯(6 mL)两步提取后,采用旋转蒸发仪蒸干,1 mL流动相溶解定容,取上清滤液20μL进样做HPLC分析。吡喹酮的血浆药物浓度在0.062 5~8 mg/L范围内,呈现良好的线性关系,相关系数r0.999。在0.062 5、1、16 mg/L的3个药物浓度水平下,吡喹酮的平均回收率均在90%以上。日内和日间变异系数小于5%。PZQ的LOD可达0.01 mg/L,LOQ为0.03mg/L。该方法的专属性强、灵敏度高,适用于水牛血浆中PZQ的药动学检测。 |
| 关 键 词： | 吡喹酮 高效液相色谱 水牛 |
| 收稿时间： | 6/6/2009 12:00:00 AM |
| 修稿时间： | 2009/12/21 0:00:00 |
A high-performance liquid chromatographic method for determination of praziquantel in water buffalo plasma |
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| SUN Yu,FAN Yu-song,FAN Jian-hua,WANG Bing-zhang,BU Shi-jin.A high-performance liquid chromatographic method for determination of praziquantel in water buffalo plasma[J].Chinese Journal of Veterinary Drug,2010,44(3):9-12. | |
| Authors: | SUN Yu FAN Yu-song FAN Jian-hua WANG Bing-zhang BU Shi-jin |
| Institution: | Veterinary Medicine of Yangzhou University, |
| Abstract: | A simple, sensitive and reproducible method based on a reversed -phase chromatography was developed for the determination of praziquantel in water buffalo plasma. Praziquantel was separated from the impurities on a C18 column （250 mm × 4.0 mm, 5 μm） ,with retention times of 10.6 rain. Ultraviolet detection was set at 220 nm. The mobile phase consisted of methanol and distilled water （70 ： 30, V/V） , running through the column at a flow rate of 0.8 mL/min. The chromatographic analysis was operated at 40℃. Sample preparation （0.5 mL plasma） was done by a single step liquid- liquid extraction with ethyl acetate. Calibration curves in plasma at the concentrations （ 0. 062 5 - 8 mg/L） were all linear with correlation coefficients higher than 0. 999. At the fortified levels of 0. 062 5,1,16 mg/L, the mean recoveries of PZQ ranged from 90% to 120%. The precision of the method based on within - day repeatability and reproducibility （ day - to - day variation ） was below 5%. Limit of detection （LOD） was 0.01 mg/L. LOQ was 0. 03 mg/L. The method was applicable to pharmaeokinetics detection of PZQ in water buffalo plasma. |
| Keywords: | Praziquantel HPLC Water buffalo |
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