| 植病生防芽孢杆菌的分离筛选与初步鉴定 |
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| 引用本文： | 张晓舟,徐剑宏,李顺鹏.植病生防芽孢杆菌的分离筛选与初步鉴定[J].土壤,2005,37(1):85-88. |
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| 作者姓名： | 张晓舟 徐剑宏 李顺鹏 |
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| 作者单位： | 南京农业大学农业部农业环境微生物工程重点开放实验室,南京,210095 |
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| 基金项目： | 江苏省自然科学基金(BK2001071)，中国科学院南京土壤研究所开放实验室基金(2025102)资助。 |
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| 摘 要： | 从山东和江苏采集的菜园土样中分离筛选得到4株芽孢杆菌B15、B110、B113和B122,它们均对植物病原真菌具有较强的拮抗能力且具有广谱性。4株芽孢杆菌可以致使黄瓜枯萎病原菌菌丝生长形态异常,出现菌丝前端膨大或变细、菌丝扭曲、分支加剧、生成大量圆珠形囊状体和原生质凝聚等异常现象。通过对4个菌株培养物形态特征的观察和一系列的生理生化实验得到其初步鉴定结果,其中B15和B113为短短芽孢杆菌(Brevibacillus brevis),B110为多粘类芽孢杆菌(Paenibacillus polymyxa),B122为枯草芽孢杆菌(Bacillus subtilis)。
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| 关 键 词： | 拮抗作用 芽孢杆菌 筛选 鉴定 生物防治 |
ISOLATION, SCREENING AND PRELIMINARY IDENTIFICATION OF PLANT PATHOGENIC FUNGI BIOCONTROL STRAINS OF BACILLUS SPP. |
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| ZHANG Xiao-zhou,XU Jian-hong and LI Shun-peng.ISOLATION, SCREENING AND PRELIMINARY IDENTIFICATION OF PLANT PATHOGENIC FUNGI BIOCONTROL STRAINS OF BACILLUS SPP.[J].Soils,2005,37(1):85-88. |
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| Authors: | ZHANG Xiao-zhou XU Jian-hong and LI Shun-peng |
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| Institution: | Key Laboratory of Microbiological Engineering of Agricultural Environment, MOA, Nanjing Agricultural University, Nanjing 210095,Key Laboratory of Microbiological Engineering of Agricultural Environment, MOA, Nanjing Agricultural University, Nanjing 210095 and Key Laboratory of Microbiological Engineering of Agricultural Environment, MOA, Nanjing Agricultural University, Nanjing 210095 |
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| Abstract: | Four strains of Bacillus spp. B15, B110, B113 and B122 were isolated from garden soil samples, which were collected from Shandong and Jiangsu Provinces. Experiments show they all had strong antagonistic effects on a broad spectrum of plant pathogenic fungi. The four strains of Bacillus spp. could induce deformed growth of hyphae of Fusarium oxysporum. With their tips bulged or attenuated, the hypha grew twisty, ramulose, yielded a large number of round cystids and their protoplasm agglomerated. Through observation of their morphological features and culture characters and a series of physiological and biochemical experiments, B15 and B113 were identified as Brevibacillus brevis, B110 as Paenibacillus polymyxa and B122 as Bacillus subtilis. |
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| Keywords: | Antagonism Bacillus spp Screening Identification Biological control |
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