Studying the extent of genetic diversity among Gossypium arboreum L. genotypes/cultivars using DNA fingerprinting |
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| Authors: | Mehboob-ur-Rahman Tahira Yasmin Nabila Tabbasam Ihsan Ullah Muhammad Asif Yusuf Zafar |
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| Institution: | (1) National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad, Pakistan |
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| Abstract: | Genetic diversity is an area of concern for sustaining crop yield. Information on genetic relatedness/diversity among Gossypium arboreum L. cultivars/genotypes is scanty. We have used random amplified polymorphic DNA (RAPD) analysis to assess the genetic divergence/relationship
among 30 genotypes/cultivars of G. arboreum. Of 45 primers surveyed, 63% were polymorphic. Out of the total number of loci amplified, 36% were polymorphic. The calculated
genetic similarity between the cultivars/genotypes was in the range of 47.05–98.73%. Two genotypes, HK-244 and Entry-17, were
the most distantly related. The average genetic relatedness among all the genotypes was 80.46%. However, most of the cultivated
varieties showed a close genetic relationship, indicating a narrow genetic base in comparison to the non-cultivated germplasm.
The calculated coefficients were used to construct a dendrogram using the unweighted pair group of arithmetic means (UPGMA)
algorithm, which grouped the genotypes/cultivars into two major and three smaller clusters. The study is the first comprehensive
analysis of the genetic diversity of G. arboreum germplasm and identifies cultivars that will be useful in extending the genetic diversity of cultivated varieties and future
genome mapping projects. |
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| Keywords: | Cultivars Genetic diversity Genotypes Gossypium arboreum L PAPD PCR |
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