| 锡盟沙地榆(Ulmus pumila L.var.sabulosa)遗传多样性的RAPD研究 |
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| 引用本文： | 李方祯,宛涛,伊卫东,蔡萍.锡盟沙地榆(Ulmus pumila L.var.sabulosa)遗传多样性的RAPD研究[J].中国农业科技导报,2008,10(3):71-76. |
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| 作者姓名： | 李方祯 宛涛 伊卫东 蔡萍 |
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| 作者单位： | （1.内蒙古农业大学生态环境学院, 呼和浩特 010018,2.锡林郭勒职业学院, 内蒙古 锡林浩特 026000） |
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| 摘 要： | 应用随机扩增多态性DNA(RAPD)标记法对内蒙古浑善达克锡盟沙地榆6个居群和对照家榆4个居群的遗传多样性进行了研究,采用14条引物共获得
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| 关 键 词： | RAPD 遗传多样性 锡盟沙地榆 |
| 收稿时间： | 2008-03-14 |
| 修稿时间： | 2008-04-10 |
RAPD Analysis on Genetic Diversity of Ulmus pumila L.var.sabulosa |
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| LI Fang-zhen,WAN Tao,YI Wei-dong,CAI Ping.RAPD Analysis on Genetic Diversity of Ulmus pumila L.var.sabulosa[J].Journal of Agricultural Science and Technology,2008,10(3):71-76. |
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| Authors: | LI Fang-zhen WAN Tao YI Wei-dong CAI Ping |
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| Institution: | (1. School of |Ecology and |Environment, Inner Mongolia |Agricultural |University, Hohhot 010018；;
2. Xilinguole Vocational College, |Inner Mongolia Xilinhaote 026000, China) |
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| Abstract: | Six populations of Ulmus pumila L.var.sabulosa and four comparison populations of Ulmus pumila were analyzed using random
amplified polymorphic DNA (RAPD) markers. Fourteen random primers amplified 118 loci, average of each primers is 8.4, 83
fragments (at a proportion of 70.34%) were polymorphic at the individual level.According to genetic distance and clustering,
Ulmus pumila L.var.sabulosa and Ulmus pumila were in two branches obviously. This indicated that Ulmus pumila L.var.sabulosa
came into being and had kept biggest genetic distance. Hence, it proves the conclusion that Ulmus pumila L.var.sabulosa is a
variant of Ulmus pumila. There are definite differences among the population of Ulmus pumila L.var.sabulosa,which are closely
related to geographical distribution. This research demonstrates applicability of RAPD in the study of genetic diversity and
systematic research of Ulmus pumila L.var.sabulosa are practicable. |
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| Keywords: | RAPD |
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