Antifungal activity of the essential oil of Zanthoxylum bungeanum and its major constituent on Fusarium sulphureum and dry rot of potato tubers |
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| Authors: | Li Xing-dong Xue Hua-li |
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| Institution: | 1. The School of Mathematics & Physics, Lanzhou Jiaotong University, Lanzhou, 730070, People’s Republic of China
2. College of Science, Gansu Agricultural University, Lanzhou, 730070, People’s Republic of China
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| Abstract: | The composition of the essential oil of Zanthoxylum bungeanum was analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 35 components, which constitute 99.10% of the oil, were identified. The major constituent was α-pinene (19.59%). Minimum inhibitory concentrations (MICs) of the essential oil and α-pinene against Fusarium sulphureum were determined to be 6.25% and 12.50%, respectively. A determination of the antifungal activity and inhibitory effect of the essential oil and α-pinene showed that the essential oil more significantly decreased cell membrane integrity and inhibited spore germination than α-pinene. Observations using scaning electron microscope revealed morphological modification including mycelium asymmetry, hyphal swelling, curling, and a secretory appearance. Ultrastructural alterations were also observed including cell empty cavity and osmiophilic granule generation. In vivo testing showed that the oil and α-pinene effectively controlled dry rot of potato inoculated with F. sulphureum. These findings indicated that the oil and α-pinene directly inhibit fungal growth, and the essential oil showed pronounced potential than α-pinene. |
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