| 添加不同碳源对零换水养殖系统中团头鲂鱼种生长、肠道生化指标和水质的影响 |
| |
| 引用本文： | 江晓浚,孙盛明,戈贤平,朱健,李冰,张成锋,缪凌鸿,董玉峰.添加不同碳源对零换水养殖系统中团头鲂鱼种生长、肠道生化指标和水质的影响[J].水产学报,2014,38(8):1113-1122. |
| |
| 作者姓名： | 江晓浚 孙盛明 戈贤平 朱健 李冰 张成锋 缪凌鸿 董玉峰 |
| |
| 作者单位： | 南京农业大学无锡渔业学院,中国水产科学研究院淡水渔业研究中心,南京农业大学无锡渔业学院,中国水产科学研究院淡水渔业研究中心,中国水产科学研究院淡水渔业研究中心,中国水产科学研究院淡水渔业研究中心 |
| |
| 基金项目： | 国家大宗淡水鱼类产业技术体系建设专项（CARS-46-14）；“十二五”国家科技支撑计划（2012BAD25B07）；中央级公益性科研院所基本科研业务费专项（2013JBFR06，2014A08XK01） |
| |
| 摘 要： | 为探究添加不同碳源物质所形成的生物絮团对团头鲂鱼种生长、消化酶以及抗氧化酶活性的影响,设计5个不同碳源物质的添加组淀粉组、葡萄糖组、蔗糖组、甜蜜素组和复合碳源组(葡萄糖∶淀粉=1∶1)],其中淀粉组为对照组,每个碳源添加组设置3个重复。每个水泥池投放团头鲂鱼种20尾,初始体质量为(36.74±0.82)g,实验期为8周。结果发现:(1)形成的生物絮团可以有效地调节水质,降低水体中的氨氮和亚硝酸盐氮水平;(2)与对照组相比,葡萄糖组团头鲂鱼种的鱼体末质量显著提高23.1%,增重率显著提高39.4%,特定生长率也显著提高23.6%,饲料系数显著降低28.1%,但存活率并没有显著差异;(3)肠道组织光镜观察表明,团头鲂鱼种肠道单层柱状上皮附近存在未消化的生物絮团;(4)添加不同碳源形成的生物絮团对团头鲂鱼种体成分没有显著的影响;(5)复合碳源组的肠道总蛋白酶的活性(3.64±0.53)U/mg显著高于对照组275.3%,淀粉酶活性显著高于对照组(淀粉组)289.2%、葡萄糖组166.7%和蔗糖组860%;(6)葡萄糖组的团头鲂超氧化物歧化酶(SOD)活性为(238.67±13.63)U/mg,显著高于对照组的SOD活性72.5%,葡萄糖组团头鲂的过氧化氢酶(CAT)酶活性为(192.31±17.06)U/mg,显著高于对照组的CAT活性40.4%,与对照组的丙二醛(MDA)水平相比,葡萄糖组、蔗糖组、甜蜜素组和复合碳源组分别显著降低了69.0%、59.7%、38%.0和48.8%。研究表明,水体中添加葡萄糖为碳源能显著提高团头鲂鱼种的生长性能和抗氧化水平,并有效改善水质。
|
| 关 键 词： | 团头鲂 碳源 生长 消化酶 抗氧化 生物絮团 |
| 收稿时间： | 2013/12/22 0:00:00 |
| 修稿时间： | 5/8/2014 12:00:00 AM |
Effects of different carbon sources on growth,intestinal biochemical parameters and water quality in the juvenile Megalobrama amblycephala cultured in zero water exchange system |
| |
| JIANG Xiaojun,SUN Shengming,GE Xianping,ZHU Jian,LI Bing,ZHANG Chengfeng,MIAO Linghong and DONG Yufeng.Effects of different carbon sources on growth,intestinal biochemical parameters and water quality in the juvenile Megalobrama amblycephala cultured in zero water exchange system[J].Journal of Fisheries of China,2014,38(8):1113-1122. |
| |
| Authors: | JIANG Xiaojun SUN Shengming GE Xianping ZHU Jian LI Bing ZHANG Chengfeng MIAO Linghong and DONG Yufeng |
| |
| Institution: | Wuxi Fishery College Nanjing Agricultural University,,Wuxi Fishery College Nanjing Agricultural University,,, |
| |
| Abstract: | An 8-week trial was conducted to investigate the effects of five carbon sources on the growth performance, intestinal biochemical parameters and water quality in the juvenile Megalobrama amblycephala by the principle of bio-floc technology(BFT).The carbon sources include starch(control), glucose, sucrose, sodium cyclamate, composite carbon(mixture of starch and glucose in proportion of 1:1).The juvenile M.amblycephala with initial body weight of(36.74±0.82)g were stocked into 15 indoor cement tanks(1 m×4 m×0.6 m)and fed commercial feed with daily normal feeding.At the initiation of the experiment, the fish were randomly chosen and sorted into 15 indoor cement tanks with 20 individuals per tank.Each experimental group was randomly assigned to triplicate tanks for a completely randomized design.The results of this study showed that bioflocs could effectively regulate water quality, as well as reduce ammonia and nitrite concentration.Final body weight, weight gain rate(WGR), specific growth rate(SGR)of glucose group were significantly higher than the other groups(P<0.05), while feed conversion ratio was significantly lower than the other groups(P<0.05).Furthermore, no significant differences(P>0.05)were observed for the survival rate and the body composition among all treatment groups.Intestine microstructure observation showed that there were some bioflocs in the brush border of intestine in juvenile M.amblycephal.The activities of protease and amylase of fish in the composite carbon group were significantly higher than control group, glucose group and sucrose group(P<0.05).The activities of superoxide dismutase and catalase of fish in the composite carbon group were 72.5% and 40.4%, which were significantly higher than those of control group, however, the malondialdehyde content was lower than that of control group(P<0.05). |
| |
| Keywords: | Megalobrama amblycephala biofloc carbon source growth digestive enzymes antioxidant enzymes |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
