|
|||||
|
|
| 8个中国梨品种S基因型的分子鉴定 | |
| 引用本文： | 梁文杰,谭晓风,张琳,曾艳玲,姜傲芳,曹玉芬,李秀根.8个中国梨品种S基因型的分子鉴定[J].果树学报,2007,24(6):757-760. |
| 作者姓名： | 梁文杰 谭晓风 张琳 曾艳玲 姜傲芳 曹玉芬 李秀根 |
| 作者单位： | 1. 中南林业科技大学经济林育种与栽培国家林业局重点实验室,长沙,410004 2. 中国农业科学院果树研究所,辽宁兴城,125100 3. 中国农业科学院郑州果树研究所,郑州,450009 |
| 基金项目： | 国家林业局重点科学技术研究计划林业新技术开发与储备专项 , 湖南省科技三项费资助项目 |
| 摘 要： | 为鉴定8个中国梨品种的S基因型,使用梨自交不亲和基因(S-RNase)特异引物"FTQQYQ"和"anti-ⅡWP-NV",对8个梨品种的基因组DNA进行特异扩增,并对扩增片段进行回收、克隆、测序。使用生物信息学软件对各序列分析和经同源性搜索分析后,确定了各品种的S基因型。结果分别是:兰州花长把为S19S22,青面为S1S18,黄面为S1S12,早蜜为S19S29,大面黄为S1S19,无子黄为S28S16,大青皮为S34S19及金锤子为S16S19。 |
| 关 键 词： | 梨 自交不亲和性 S基因型 鉴定 |
| 文章编号： | 1009-9980（2007）06-757-04 |
| 修稿时间： | 2007-04-18 |
Molecular identification of S-genotypes of Chinese pear cultivars |
|
| LIANG Wen-jie,TAN Xiao-feng,ZHANG Lin,ZENG Yan-ling,JIANG Ao-fang,CAO Yu-fen,LI Xiu-gen.Molecular identification of S-genotypes of Chinese pear cultivars[J].Journal of Fruit Science,2007,24(6):757-760. | |
| Authors: | LIANG Wen-jie TAN Xiao-feng ZHANG Lin ZENG Yan-ling JIANG Ao-fang CAO Yu-fen LI Xiu-gen |
| Abstract: | Using a pair of primers specific to pear S-RNases,PCR amplification from genomic DNAs of eight Chinese pear cultivars were carried out. Then the PCR products were extracted,cloned and sequenced. The amplified fragments were assigned to their respective S-RNases by Blast analysis. The S-genotypes of the eight tested self-incompatible cultivars were identified as follows: Lanzhou Huachangba (S19S22),Qingmian (S1S18),Huangmian (S1S12),Zaomi (S19S29),Damianhuang(S1S19),Wuzihuang (S28S16),Daqingpi (S34S19) and Jinchuizi (S16S19). |
| Keywords: | Pyrus Self-incompatibility S-genotype Identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |