| 筛选一组用于鉴定小麦缺体-四体真实性的SSR分子标记 |
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| 引用本文： | 卫 笑,杨 靖,孙海燕,曹银萍,巴爱丽,李友勇.筛选一组用于鉴定小麦缺体-四体真实性的SSR分子标记[J].中国农学通报,2018,34(20):47-54. |
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| 作者姓名： | 卫 笑 杨 靖 孙海燕 曹银萍 巴爱丽 李友勇 |
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| 作者单位： | 河南科技学院/现代生物育种河南省协同创新中心,河南科技学院/现代生物育种河南省协同创新中心,河南科技学院/现代生物育种河南省协同创新中心,河南科技学院/现代生物育种河南省协同创新中心,河南科技学院/现代生物育种河南省协同创新中心,河南科技学院/现代生物育种河南省协同创新中心 |
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| 基金项目： | 河南省基础与前沿计划重点项目(122300410011,162300410136)。 |
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| 摘 要： | 目的]旨在筛选一组数量较大,可靠性较高的SSR分子标记,用于辅助鉴定小麦缺体-四体的真实性。方法]方法是在小麦SSR分子标记图谱上,选取单一位点标记引物,并在中国春和已鉴定的缺体-四体DNA中扩增检测,筛选带型清晰,易识别的单扩增产物标记。结果]结果表明,在小麦21个连锁群上共选择标记150个,长短臂均有分布,最终筛选出标记67个,这些标记在中国春和所有非连锁的缺体-四体中扩增为阳性,在对应的连锁的缺体-四体中扩增为阴性,扩增产物为单条带,每个连锁群上最少3个,最多5个。结论]该套引物,带型清晰,易识别,单一位点,适用于所有中国春小麦缺体-四体及其他非整倍体的真实性鉴定。
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| 关 键 词： | Z指数 Z指数 气象干旱 时空分布 聊城地区 |
| 收稿时间： | 2017/6/28 0:00:00 |
| 修稿时间： | 2018/6/19 0:00:00 |
Screening of SSR Molecular Markers for Identifying the Authenticity of Nulli-Tetrasomes of Wheat |
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| Abstract: | The aim is to screen a group of SSR (simple sequence repeat) molecular markers with relatively high quantity and reliability which could identify the authenticity of nulli-tetrasomes lines of wheat. The single locus makers on the SSR molecular marker map of wheat were selected, and then the makers were amplified in DNA of Chinese Spring and Chinese Spring nulli-tetrasomes lines. And then the primers that produced clear and easy-identified band were screened out. The results showed that a total of 150 SSR markers on 21 linkage maps of wheat covering the long and short arms of each chromosome were selected. At last, 67 markers were selected which showed positive amplification in Chinese Spring and non- linkage nulli- tetrasomes, and negative amplification in the corresponding linkage nulli-tetrasomes. The production was the single band. This set of primers distributed evenly over all chromosomes of wheat genome, at least 3, up to 5 on each chromosome, and their bands were single and easily identified, and could be efficiently used for identifying the authenticity of Chinese Spring nulli-tetrasomes lines and other aneuploidy of wheat. |
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| Keywords: | wheat SSR molecular markers Chinese spring nulli-tetrasomes authenticity identification |
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