Comparison of symbiotic effectiveness of Rhizobium sp. with positive hydrogen-uptake activity compared with negative mutants in relation to nitrogen fixation in mungbean (Vigna radiata L.) |
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| Authors: | Mohd Yousuf Zargar R S Kahlon |
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| Institution: | (1) Section of Microbiology, SK University of Agricultural Sciences and Technology, 191121 Shalimar, Kashmir, India;(2) Present address: P. O. Box 224, G.P.O., 190001 Srinagar, Kashmir, India;(3) Department of Microbiology, Punjab Agricultural University, 141004 Ludhiana, India |
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| Abstract: | H2 uptake activity was well distributed in Rhizobium sp. strains isolated from nodules of mung-bean (Vigna radiata L.). Two effective strains, RMP1 und RMP2, exhibiting significantly higher H2 uptake activity were subjected to mutagenesis with nitrosoguanidine. The respective mutation frequencies were 0.18 and 0.19%. Three Hup- mutants each of RMP1 und RMP2 were compared with the wild-type parent strains under pot culture experiments to evaluate the significance of the H2 uptake system in biological N2 fixation. Nodulation capabilities, plant growth characteristics, and the chlorophyll content of the leaves were significantly reduced in the plants treated with Hup- mutants. Nitrogenase activity in Hup- nodules was reduced by 8–41%. Similarly, N accumulation was also reduced singificantly. |
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| Keywords: | Mungbean Vigna radiata Nitrogen fixation Hydrogen uptake Mutation Nitrosoguanidine |
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